Evaluation of charged molecules content of plant polysaccharides by aqueous size exclusion chromatography

Authors

  • Stefan Willför Professor, Åbo Akademi University, Process Chemistry Centre, Turku/Åbo, Finland, swillfor@abo.fi
  • Azamat S. Boymirzaev Docent, Namangan Institute of Engineering and Technology, Department of Physics, Uzbekistan. +998 93 4011255 azamatsb@gmail.com
  • Andrey Pranovich Docent, Åbo Akademi University, Process Chemistry Centre, Turku/Åbo, Finland, +358 2 215 4987 apranovi@abo.fi

DOI:

https://doi.org/10.17308/sorpchrom.2017.17/429

Keywords:

size exclusion chromatography, molar mass, heteropolysaccharides, polyelectrolyte expansion, concentration effects

Abstract

Aqueous size-exclusion chromatography (SEC) equipped with refractive index (RI) and multiangle laser light scattering (MALLS) detectors were applied to evaluation of charged molecules content in a series of polysaccharide derivatives with different average molar mass (MM) values. It was shown that a minor amount of residual charged groups in the polysaccharide chains caused a polyelectrolyte expansion effect and neutral fractions of birch xylans, larch arabinogalactans (AG) and galactoglucomannans (GGM) were separated from neutral molecules in various concentrations of the injected samples

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Published

2018-02-22

Issue

Vol. 17 No. 4 (2017): Sorption and chromatographic processes

Section

Статьи

How to Cite

Evaluation of charged molecules content of plant polysaccharides by aqueous size exclusion chromatography. (2018). Sorbtsionnye I Khromatograficheskie Protsessy, 17(4), 689-694. https://doi.org/10.17308/sorpchrom.2017.17/429