Identification of oil markers – indicators of biodegradation by HPLC-MS
DOI:
https://doi.org/10.17308/sorpchrom.2025.25/13578Keywords:
oil markers, biodegradation, HPLC-MS, calibration schedule, mass spectrumAbstract
Oil markers are special chemical substances added in small quantities to the original oil for identification, product tracking at various stages of its life cycle and assessment of biodegradation during storage. Existing methods for marker determination mainly involve the use of HPLC, which gives acceptable results if the solutions do not contain foreign compounds similar to the desired markers in their chemical nature, which can have the same retention time with the determined label, which significantly complicates the use of the technique.
Due to the relevance of the problem of determining oil markers, work has been carried out to identify compounds that can be used as indicators of biodegradation, using a combined HPLC-MS method. In this case, in addition to the retention time, the criterion for the presence of the marker will also be its molecular weight.
The developed technique is based on the individual characteristics of 5 artificial markers, the concentrations of which varied within 5-100 ppm, necessary for constructing a calibration graph with a high linearity criterion. The equations of straight lines are determined. The reliability of the developed method was verified by chromatography of a mixture of labels in a "blind" sample.
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