Research of chromatographic behavior of some derivatives of benzimidazole in the conditions of reverse phase HPLC

Abstract

At present, there are quite a number of works devoted to the study of biologically active substances
(BAS) by high performance liquid chromatography. The authors examine the impact of structure of BAS on
the retention using different stationary and mobile phases. They study various correlation models of the type
«structure - retention-biological activity», that allows to predict the kind of biological activity, depending on
the structure of BAS and on the basis of these data directed synthesis of compounds. When studying the effect
of eluent composition on the substances retention in the HPLC researchers use semi-empirical models:
Snyder–Soczewinski, Scott–Kucera, Eltekov, Lanin-Nikitin. The aim of this work was to study the effect of
the structure of newly synthesized benzimidazole derivatives and composition of aqueous acetonitrile eluent
on the chromatographic retention by octadecyl silica gel. We studied some of the features of the chromatographic
behavior of newly synthesized benzimidazoles on octadecyl silica gel from aqueous acetonitrile eluents.
It is shown that the nature and position of substituents substantially affect retention and separation selectivity.
This samples of benzimidazole derivatives described satisfactorily by a Snyder–Soczewinski model.
The data obtained can be used to predict the retention of substances belonging to that class of organic compounds.