Use of lithium perchlorate in reversed-phase high performance liquid chromatography of amino compounds

Authors

  • Irina N. Azarova PhD, Chemist expert, EcoNova, Ltd., Institute of Chromatography, Novosibirsk, Russia, iazarova@mail.ru
  • Grigory I. Baram Dr. Sci. (Chem.), Deputy Director General on Science, EcoNova, Ltd., Institute of Chromatography, Novosibirsk, Russia

Keywords:

RP-HPLC; ion-pairing agent; lithium perchlorate; amino compounds.

Abstract

The influence of lithium perchlorate as a mobile phase (MP) component on chromatographic
behavior of amino compounds in reversed-phase high performance liquid chromatography (RP-HPLC) was
studied. It was shown that the addition of LiClO4 in MP (pH 2) in both isocratic and gradient elution mode
leads to the increase of retention for protonated bases and to the decrease of their peak asymmetry and peak
widths. It was found that the maximal effect observed at concentrations of LiClO4 in MP ≥ 0.2 M.

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Published

2019-11-18

Issue

Vol. 14 No. 1 (2014): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

Use of lithium perchlorate in reversed-phase high performance liquid chromatography of amino compounds. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 14(1). https://journals.vsu.ru/sorpchrom/article/view/1432