Extraction technologies for clinical HPLC analysis of serum progesterone, 17α-hydroxyprogesterone and synthetic progestin’s

Authors

  • Alexey A. Dutov МD, Senior Research Laboratory of Experimental and Clinical Biochemistry and Immunology, Research Institute of Molecular Medicine of the Medical Academy.Chita, e-mail: dutovaa@yandex.ru
  • Denis A. Nikitin head of the laboratory of chemical analysis of the Zabaikalsky State University, Chita, e-mail: nikitinnd@gmail.com
  • Pavel P. Tereshkov PhD, Head laboratory of experimental and clinical biochemistry and immunology Research Institute of molecular medicine of the Medical Academy.Chita
  • Alexander D. Kolesnikov PhD, obstetriciangynecologist of the highest category. Road Clinical Hospital at station Chita-2, OAO RZD
  • Anna V. Sverkunova Post-graduate student of chemistry the Zabaikalsky State University, Chita
  • Anastasia V. Martynova Post-graduate student Department of Chemistry Zabaikalsky State University, Chita
  • Olga N. Konovalova Post-graduate student Department of Chemistry, Zabaikalsky State University, Chita
  • Ekaterina N. Fedorova Post-graduate student Department of Chemistry, Zabaikalsky State University, Chita
  • Julia L. Lukyanova a physician ordinator Department of Neurology, Medical Academy, Chita
  • Alena V. Yermolina physician ophthalmologist Regional Hospital № 2, Chita

Keywords:

17a-hydroxyprogesterone, progesterone, liquid-liquid extraction (LLE), solid-phase extraction (SPE), hyper cross-linked polystyrene, HPLC.

Abstract

The methods of liquid-liquid extraction (LLE) and solid-phase extraction (SPE) from human serum
for 17a-hydroxyprogesterone and progesterone, as well as, synthetic progestin's are developed. The analysis
carried out by means of conventional reversed-phase HPLC with UV detection. LLE made by mean of
pentane – dichloromethane/chloroform, SPE on cartridges packed 30 mg hyper cross-linked polystyrene.
Simplicity, reproducibility both sufficient selectivity and sensitivity of a method, allow to use it in a clinical
practice for an estimation of the hormonal status at women, including receiving synthetic steroid drugs.

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Published

2019-11-19

Issue

Vol. 14 No. 2 (2014): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

Extraction technologies for clinical HPLC analysis of serum progesterone, 17α-hydroxyprogesterone and synthetic progestin’s. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 14(2). https://journals.vsu.ru/sorpchrom/article/view/1471

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