Synthesis and immobilization of liposomes on the basis of phosphatidylcholine and palmitic acid on the electrode surface of the piezoelectric sensor
Keywords:
liposomes, phosphatidylcholine, palmitic acid, avidin, biotin, piezoelectric sensor, immobilization.Abstract
The conditions of synthesizing normal liposomes of the fixed size on the basis of
phosphatidylcholine and palmitic acid were investigated and the regularity of their immobilization on the
surface of electrodes of piezoelectric sensors are studied. It is shown that the dehydration and rehydration
method with the subsequent ultrasonic dispergating during 1 h in the presence of Triton X 100 in the mass
relation to lipid 1:800 is most suitable for receiving monodisperse fractions of liposomes with the diameter
of 61 ± 4 nm. Various technologies of fixing liposomes or biotinylated liposomes on the surface of the
sensor are compared and the quality of the obtained coverings is investigated by QCM and AFM methods.
It is shown that optimum values of mass (1,47 ± 0,03 mkg), surface roughnesses (124 ± 13 nn) and the
maximum stability (for 30 days) characterize flat layers of the liposomes immobilized on the silane substrate
activated by glutaraldehyde through the avidin-biotinvy complex. The obtained results can be used when
forming the receptor layer of piezoelectric sensors and will allow to expand the circle of the defined
connections significantly.
