Dependence of chromatographic retention indices on a ratio of target analytes and reference components. testing of different columns

Abstract

Contemporary recommendations on determination of chromatographic retention indices (RI) imply
no control of relative amounts of target analytes and reference compounds. Whereas, RI dependence on such
ratios seems to be a principal factor which determines their interlaboratory reproducibility. Hence, the
usefulness of these analytical parameters in identification of unknown analytes depends on this effect, as
well.
The dependence under consideration permits us to distinguish partition and adsorption mechanisms
of chromatographic separation. Using this feature, the simple method to evaluate the aging of
chromatographic columns in reversed phase HPLC is proposed first time.