Chromatographic isolation and purification of low-molecular-weight peptide complexes from sea-urchin eggs

Authors

  • P. Yu., Morozova student of the chair of biochemistry, St Petersburg State University, St Petersburg., e-mail: aravenko_polina@yahoo.com
  • D. V. Zhilinski graduate student, Institute of bioregulation and gerontology of the North- Western Branch of the Russian Academy of Medical Sciences , St Petersburg, St Petersburg
  • A. Yu. Morozova student of the chair of biochemistry, St Petersburg State University, St Petersburg.
  • A. A. Kolobov engineer of the chair of biochemistry, St Petersburg State University, St Petersburg.
  • A. Yu. Solovyev senior researcher, Institute of macromolecular compounds of RAS, St Petersburg

Keywords:

low molecular weight peptides, sea urchin eggs, HPLC, HPTLC, organotypic tissue culture.

Abstract

Cross-flow microfiltration, solid phase extraction, reversed-phase high performance liquid
chromatography and high performance were used for separation and purification of low molecular weight
peptides from the sea urchins eggs Strongylocentrotus pallidus and Strongylocentrotus droebachiensis. It
was shown that the isolated peptides have pronounced stimulating activity with respect to tissues of
mesodermal origin (spleen, heart, liver).

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Published

2019-11-26

Issue

Vol. 11 No. 6 (2011): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

Chromatographic isolation and purification of low-molecular-weight peptide complexes from sea-urchin eggs. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 11(6). https://journals.vsu.ru/sorpchrom/article/view/1993