Chromatographic behavior of amino acid derivatives obtained with o-phthalic aldehyde and various nucleophilic agents

Abstract

The chromatographic separation of major amino acids on the «Zorbax Eclipse AAA»
column after derivatization with o- phthaldialdehyde and various thiol compounds was studied. The
advantage of using N-isobutiryl-L-cysteine was established. Using this reagent allows one to avoid the
decrease of separation selectivity while following the technique suggested by column producer. The
correlations between the capacity factor and hydrophobicity coefficient providing the ability to predict the
order of sorbates elution were obtained