Usage of phenol - chloroform extraction and ion-exchange chromatography for analysis of expression of corn scutellum genes CYT-MDH and MT-MDH

Authors

  • Vasily N. Popov professor, Head of Dept. Genetics, Cytology and Bioengenering, Voronezh State University, Vorornezh, e-mail pvn@bio.vsu.ru
  • Elena V. Maltseva PhD Student, Voronezh State University, Vorornezh
  • Olga I. Grabelnykh PhD, senior scientist, SIPBP RAS, Irkutsk
  • Anastasia S. Strobykina PhD Student, Kazan (Povolzhsky) federal university, Kazan
  • Ekaterina B. Gorsheneva PhD Student, Tambov state university, Tambov
  • Olga P. Lebedeva PhD, Docent, Belgorod state university, Belgorod, e-mail safonova2@yandex.ru
  • Oleg Yu. Fomenko PhD, Head of the Lab, All- Russia research veterinary institute of a pathology, pharmacology and therapy, Vorornezh, e-mail fomenych2001@mail.ru
  • Еlena V. Semenova PhD, Docent, Voronezh state medical academy, Voronezh, e-mail maluzhenko@gmail.com
  • Yulia V. Chekmeneva PhD, Assistant, Voronezh Forestry Academy, Vorornezh
  • Nikita A. Karpechenko PhD Student, Research institute of wood genetics and selections, Vorornezh, e-mail nikitakarpechenko@rambler.ru

Keywords:

Phenol-chloroform extraction, malate dehydrogenase, expression.

Abstract

Us is rotined, that for mRNA isolation Phenol - Chloroform extraction in combination with an
ion exchange on an anionite AV - 17 is possible. In the given activity the attempt is made to analyze
during an ontogenesis of plants there is a regulation of metabolic pathways on molecular level. The
differential expression of a malate dehydrogenase gene shows available of several forms of an enzyme,
that can be one of gears of a regulation of metabolic pathes in a vegetative cell

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Published

2019-11-27

Issue

Vol. 11 No. 6 (2011): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

Usage of phenol - chloroform extraction and ion-exchange chromatography for analysis of expression of corn scutellum genes CYT-MDH and MT-MDH. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 11(6). https://journals.vsu.ru/sorpchrom/article/view/2004

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