Application of ion–exchange chromatography for the purification of glutathione reductase and glutathione peroxidase from rat brain at norm conditions and under cerebral ischemia-reperfusion

Authors

  • Olga V. Suhoveeva the post-graduate student of the Department of Medical Biochemistry and Microbiology, Biology and Soil Science Faculty of Voronezh State University, Voronezh, е-mail: ooleeenka@mail.ru
  • Tatyana N. Popova Dr. of Biology, Prof., the Head of the Department of Medical Biochemistry and Microbiology, Biology and Soil Science Faculty, Voronezh State University, Voronezh
  • Alexander A. Agarkov Dr. of Biology, the Assistant of the Department of Medical Biochemistry and Microbiology, Biology and Soil Science Faculty of Voronezh State University, Voronezh
  • Natalia A. Dedikova The undergraduate student of the Department of Medical Biochemistry and Microbiology, Biology and Soil Science Faculty of Voronezh State University, Voronezh

Keywords:

rat, brain, ischemia-reperfusion, free radical oxidation, glutathione reductase, glutathione peroxidase.

Abstract

Using fractionation by ammonium sulfate, gel-filtration on Sephadex G-25, ion-exchange
chromatography on DEAE-cellulose purified enzyme preparations of glutathione peroxidase (GP; EC
1.11.1.9) and glutathione reductase (GR; EC 1.6.4.2) from brain of rat of control group with specific
activity 0.37, 0.21 E/mg of protein and from brain of animals under cerebral ischemia-reperfusion with
specific activity 0.62, 0.69 E/mg of protein have been obtained; the degree of purification was 34.0, 30.0,
and 44.5, 45.0 accordingly. Using of the purified enzyme preparations kinetic properties of enzymes have
been established

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Published

2019-11-27

Issue

Vol. 11 No. 6 (2011): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

Application of ion–exchange chromatography for the purification of glutathione reductase and glutathione peroxidase from rat brain at norm conditions and under cerebral ischemia-reperfusion. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 11(6). https://journals.vsu.ru/sorpchrom/article/view/2006

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