Using ion exchange chromatography to obtain the succinate dehydrogenaseof bacteria Sphaerotilus natans strain D-507

Authors

  • Thi Loan Vu graduate student, Department of Biochemistry and Physiology, Voronezh State University, Voronezh, e-mail: bc366@bio.vsu.ru
  • Natalia V. Selivanova graduate student, Department of Biochemistry and Physiology, Voronezh State University, Voronezh
  • Alexander T. Eprintsev Doctor of Biology, Department of Biochemistry and Physiology, Voronezh State University, Voronezh
  • Dmitry N. Fedorin Ph.D of Biology, Department of Biochemistry and Physiology, Voronezh State University, Voronezh

Keywords:

succinate dehydrogenase, isoforms, ion-exchange chromatography, electrophoresis, Sphaerotilus natans.

Abstract

Abstract: Using the method of ion-exchange chromatography on a column of DEAE-cellulose
were electrophoretically homogeneous preparations of succinate dehydrogenase (SDH) from colorless
sulfur bacteria with a specific activity of 22 units/mg protein (for the first isoform) and 14.75 units/mg
protein (for the second isoform); yield 15.52%, and 10, 41%, degree of purification 81.48 and 54.63
respectively.

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Published

2019-11-27

Issue

Vol. 11 No. 6 (2011): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

Using ion exchange chromatography to obtain the succinate dehydrogenaseof bacteria Sphaerotilus natans strain D-507. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 11(6). https://journals.vsu.ru/sorpchrom/article/view/2008

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