Optimization of several flavonoids separation by TLC

Abstract

Thin-layer chromatography retention parameters are defined for several flavonoids (rutin,
naringin, (+)-catechin, quercetin) in systems based on amylol and ethyl acetate as the elution system. The
change in the chromatographic parameters of flavonoids is considered, depending on the polarity of the
eluent by Snyder, Hildebrand parameter, the dielectric constant of solvents. The nonmonotonic variation
of the flavonoids retention demonstrates necessity to consider not only the polarity of the mobile phase,
but also the nature of the solvent that affects the interaction of flavonoids with the stationary phase.
Composition of the solvent system does not significantly affect on the retention of aglycones of
flavonoids (quercetin and (+)-catechin) if normal silica uses as a stationary phase. Replacement of the
stationary phase for polyamide leads to an inversion of the order of mass transfer for glicosidic for and
aglycones