Chromatography different types application for purification of glutathione peroxidase and glutathione reductase from pathologically changed rat liver

Authors

  • Olga A. Safonova Dr. of Biology, the Assistant of the Department of Medical Biochemistry and Microbiology, Biology and Soil Science Faculty of Voronezh State University, Voronezh, е-mail: safonova@bio.vsu.ru
  • Konstantin K. Shulgin Dr. of Biology, the Assistant of the Department of Medical Biochemistry and Microbiology, Biology and Soil Science Faculty of Voronezh State University, Voronezh, е-mail: kkshulgin@mail.ru
  • Alexander A. Agarkov Dr. of Biology, the Assistant of the Department of Medical Biochemistry and Microbiology, Biology and Soil Science Faculty of Voronezh State University, Voronezh
  • Tatyana N. Popova Dr. of Biology, Prof., the Head of the Department of Medical Biochemistry and Microbiology, Biology and Soil Science Faculty, Voronezh State University, Voronezh
  • Laila Saidi the Post-graduate Student of the Department of Medical Biochemistry and Microbiology, Biology and Soil Science Faculty of Voronezh State University, Voronezh

Keywords:

glutathione peroxidase, glutathione reductase, chromatographical methods of purification, rat liver, toxic hepatitis, hyperthyroidism.

Abstract

Glutathione peroxidase (GP; EC 1.11.1.9) has been purified in 39.2; 44.1 и 31.5 times from liver
of control group rats, animals with experimental toxic hepatitis (ETH) and hyperthyroidism (EHT)
accordingly by gel-filtration on Sephadex G-25 and ion-exchange chromatography on DEAE-Cellulose.
Purified in 56.4; 48.8 и 62.3 times glutathione reductase (GR; EC 1.6.4.2) from rat liver at norm, under
ETH and EHT has been obtained by ammonium sulfate fractionation, gel-filtration on Sephadex G-25 and
ion-exchange chromatography on DEAE-Cellulose. Using purified enzyme preparations the dependence
of GP- and GR-reaction rate from substrate and coenzyme concentration, medium pH, magnesium ions
concentration and also citrate influence on the enzymes functioning have been investigated.

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Published

2019-11-27

Issue

Vol. 11 No. 6 (2011): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

Chromatography different types application for purification of glutathione peroxidase and glutathione reductase from pathologically changed rat liver. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 11(6). https://journals.vsu.ru/sorpchrom/article/view/2014

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