Upscaling of protein frontal chromatography processes on carboxylic monolithic media

Abstract

Upscaling of frontal chromatographic processes using the monolithic 8 ml column on basis of
glycidylmethacrylate-ethylenglycoldimethacrylate (GMA-EDMA) copolymer was performed. By means
of carboxyimethylation of the polymer surface the cation-exchanger with the total exchange capacity 0.2
mg-equiv/g was obtained. Experiments with dynamic sorption of the model cytochrom c - BSA or
cytochrom c – fibrinogen mixtures showed the increase of the sorption selectivity for the larger proteins
in comparison to the monolithic 0.3 ml disks. Results obtained for the model mixtures were used in the
process of plasma proteins separation.