Group system of nonequilibrium chromatography regimes

Abstract

The Systematization of laws of inequilibrium elution liquid chromatography is completed. It can be used
when the kinetic of the interphase mass changing in the column (for each of the components) is limited by inner
diffusion and linear isotherm. This systematization is based on the knowledge about different regimes of the
separated component’s zones. Each component’s zone moves in the three regimes (quasiequilibrium, inequilibrium
and intermediate) having different rate of interphase inequilibration in the column and consequently different
qualities. Separation of the two components is possible in one of the fourteen regimes of chromatography. The certain
chromatography regime is realized within the strict limits of the following parameters of the dynamic system physical
and chemical (diffusion and equilibrium coefficients) and hydro-structural (the velocity of elution, the sizes of porous
granules and the column). Theoretical analysis of all the multitude of the limits revealed the existence of separate
groups of chromatography regimes, successions of alternation of chromatography regimes in each of them during
continuous variation of the system’s parameters (“chromatography code”) and made it possible to systematize
elements consisting of regimes, codes and groups as a united “group system of chromatography regimes” (GSCR).
The consequences following this group system and its significance for theory and practical use of chromatography
are discussed as well as the prediction of its new possibilities of chromatography