The chromatographic and electrophoretic determination of catecholamines, metanephrines and 3,4-dihydroxyphenylalanine in urine and blood plasma

Authors

  • Alla A. Sidorova Dr.Sc.Chem. Engineer CSU Analytical Spectrometry St. Petersburg state polytechnic university, St. Petersburg, e-mail: csu@delfa.net
  • Ludmila A. Kartsova Dr.Sc.Chem, Professor of organic chemistry department of chemical faculty, St. Petersburg state university, St. Petersburg

Keywords:

catecholamines, methanephrine, 3,4-dihydroxyphenylalanine, HPLC, capillary electrophoresis, solid-phase extraction.

Abstract

Various procedures of sample preparation of biological objects (urine and blood plasma),
including SPE with aluminum oxide and strong cation exchange sorbent were investigated. The efficiency
of catecholamines, metanephrines and 3,4-dihydrohyphenylalanine extraction was 90%. The capillary
zone electrophoresis with UV detection and reversed-phase high performance liquid chromatography with
amperometric detection were compared for catecholamines and metanephrines analysis in urine and blood
plasma.

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Published

2019-11-27

Issue

Vol. 10 No. 4 (2010): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

The chromatographic and electrophoretic determination of catecholamines, metanephrines and 3,4-dihydroxyphenylalanine in urine and blood plasma. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 10(4). https://journals.vsu.ru/sorpchrom/article/view/2074

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