Application of ion-exchange chromatography to obtain highly purified preparations aconitate hydratase

Authors

  • Amin Alnasser graduate student, Department of Biochemistry and Cell Physiology, Voronezh State University, Voronezh
  • Marina V. Zajchikova graduate student, Department of Biochemistry and Cell Physiology, Voronezh State University, Voronezh
  • Julia A. Stolnikova student, Department of Biochemistry and Cell Physiology, Voronezh State University, Voronezh, e-mail: marinazajchikova@yandex.ru
  • Alexander T. Eprintsev Doctor of Biology, Department of Biochemistry and Cell Physiology, Voronezh State University, Voronezh

Keywords:

aconitate hydratase, aconitase, isoforms, ion-exchange chromatography, electrophoresis, maize scutellum, hepatocytes.

Abstract

The dynamic of aconitase activity from maize scutellum and rat liver was studied. Using gelchromatography
on DEAE-cellulose and DEAE- sepharose let to obtain cytoplasmic form of enzyme in
highly purified electrophoretically homogeneous condition with specific activity of 2,4 units/mg protein
(for plant object) and 2,06 units/mg protein (for animal object). Kinetic and physico-chemical properties
of aconitase were investigated.

Downloads

Download data is not yet available.

References

Downloads

Published

2019-11-28

Issue

Vol. 10 No. 6 (2010): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

Application of ion-exchange chromatography to obtain highly purified preparations aconitate hydratase. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 10(6). https://journals.vsu.ru/sorpchrom/article/view/2133

Most read articles by the same author(s)