Determination of some antihypertensive drugs in blood plasma by high performance liquid chromatography / tandem mass spectrometry in the analysis of acute poisonings

Abstract

The selective, sensitive and reproducible method for the quantification of atenolol, bisoprolol,
verapamil, metoprolol, nifedipine, enalapril, and propranolol in human blood plasma by reverse phase high
Мельников и др. / Сорбционные и хроматографические процессы. 2015. Т. 15. Вып. 2
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performance liquid chromatography with tandem mass spectrometry (HPLC-MS / MS) was developed.
Electrospray (ESI) and chemical ionization at atmospheric pressure (APCI) were simultaneously used in the
ion source. The detection was performed by multiple reaction monitoring (MRM) in positive ionization
mode. Sample preparation was performed by plasma protein precipitation with acetonitrile. Calculations in
quantitative determination were carried out by external calibration. Calibration curves were linear in the
concentration range of 10-5000 ng/ml. The developed method may be used for the diagnosis of acute
poisoning by antihypertensive drugs.