The study of amino acid composition of herb Scabiosa ochroleuca L. by HPLC

Abstract

Purpose of work was to analyze the amino acid composition of the herb Scabiosa ochroleuca L. family Dipsacaceae by high performance liquid chromatography (HPLC). The object of the study was air-dried chopped herb Scabiosa ochroleuca L., harvested in the flowering phase. The presence of amino acids set qualitative reactions. To study the content of amino acids was applied the method of high performance liquid chromatography (HPLC). The contents of amino acids were determined using amino acid analyzer (Amino Acid Analyzer AAA 400).

Qualitative reactions proved the presence of amino acids. By HPLC revealed the existence of 15 amino acids (12 aliphatic; 1 heterocyclic and 2 aromatic amino acids, which is consistent with the literature data on the content of amino acids in plants). 7 acids are essential (threonine, valine, methionine, isoleucine, leucine, phenylalanine, lysine). The content of amino acids makes up 4,94%, including essential amino acids of 1,66%. At present the predominant concentration of glutamic acid (0,63%). The amino acid composition of the investigated plants was studied for the first time. The results indicate the prospects of using plants as a source of amino acids. In combination with other groups of biologically active substances (phenolic compounds, polysaccharides, macro - and microelements and other) it emphasizes the therapeutic importance of herbs Scabiosa ochroleuca L. and enables the development of new affordable domestic medicines combined action on the basis of promising species of medicinal plants.