Features of determining the individual biologically active derivatives aniline in a thin layer of the reversed-phase sorbent

Abstract

Peculiarities of chromatographic behaviour the individual biologically active derivatives aniline in a thin layer of the reversed-phase sorbent (models of grafted phase C14-C15) have been studied. Objects of the study were as follows: 4-nitro-3-(trifluoromethyl)aniline, aniline, 4-nitroaniline, flutamide, 2-methyl-N-(4amino-3-(trifluoromethyl) phenyl) propanamide and N-(4-acetamino-3-(trifluoromethyl) phenyl)acetamide. A possibility of using the offered chromatographing conditions for the identification of the objects of investigation in the biological materials has been shown. Calculated additional chromatographic parameters describing the conditions of the determination of analytes by reverse-phase TLC. The method of determination discussed aniline derivatives in biological material by reverse-phase TLC using a mobile phase of water-acetone (5: 5).