About chromatographic behavior of flavonoids in reversed-phase HPLC
Keywords:
flavonoids, retention, particularities, reversed-phase HPLC, separation map, effectivity map.Abstract
It has been shown that particularities of different flavonoids retention in reversed-phase HPLC caused by basic structure alteration or by introduction (or withdrawal) of functional groups may be revealed by separation map analysis constructed by relative retention analysis method. It has been proposed that the particularities are connected with no identical sorption mechanisms because of the molecule steric structure differences; the phenomenon may be detected by analysis of the chromatographic effectivity map.









