The use of reverse phase chromatography methods for the identification and quantification of flutamide and similar structures of substances in biological fluids
Keywords:
flutamide, synthesis intermediates, metabolites, TLC, column chromatography, HPLC, biological fluid.Abstract
The object of the study was antitumor drug flutamide (2-methyl-N- (4-nitro-3- (trifluoromethyl)
phenyl) propanamide) having significant toxicity and a number of structurally related compounds. As discussed
TLC analytical methods, liquid chromatography, low pressure column and HPLC.
The features of the chromatographic activity flutamide, 4-nitro-3-trifluoromethylaniline, 2-methyl-
N- (4-amino-3- (trifluoromethyl) phenyl) propanamide, and 2-methyl-N- (4-amino-3- (trifluoromethyl )
phenyl) propanamide in thin layers and columns obraschёnnofazovyh sorbents using mobile phases based on
water and buffer solutions with addition of 20-80% (by volume) of organic modifiers. The parameters of this
group of substances Chromatographic methods of TLC, column chromatography, HPLC and low pressure.
Best mobile phases for thin layer chromatography in silica gel CTX-1A, modified with C14 to C15 alkanes,
were water-acetonitrile (60:40) and 20 mM KH2PO4 buffer solution (pH 3.5) - acetonitrile (65:35) in the
sorbent column «Silasorb C-18»- water - acetonitrile (40:60), column sorbent LiChrospher RP - 20 mM
KH2PO4 solution (pH 5.3) - acetonitrile (65:35). A technique to identify and quantify flutamide, and its precursor the synthesis of two metabolites in biological fluids by applying reverse phase chromatography methods.









