The use of reverse phase chromatography methods for the identification and quantification of flutamide and similar structures of substances in biological fluids

Abstract

The object of the study was antitumor drug flutamide (2-methyl-N- (4-nitro-3- (trifluoromethyl)
phenyl) propanamide) having significant toxicity and a number of structurally related compounds. As discussed
TLC analytical methods, liquid chromatography, low pressure column and HPLC.
The features of the chromatographic activity flutamide, 4-nitro-3-trifluoromethylaniline, 2-methyl-
N- (4-amino-3- (trifluoromethyl) phenyl) propanamide, and 2-methyl-N- (4-amino-3- (trifluoromethyl )
phenyl) propanamide in thin layers and columns obraschёnnofazovyh sorbents using mobile phases based on
water and buffer solutions with addition of 20-80% (by volume) of organic modifiers. The parameters of this
group of substances Chromatographic methods of TLC, column chromatography, HPLC and low pressure.
Best mobile phases for thin layer chromatography in silica gel CTX-1A, modified with C14 to C15 alkanes,
were water-acetonitrile (60:40) and 20 mM KH2PO4 buffer solution (pH 3.5) - acetonitrile (65:35) in the
sorbent column «Silasorb C-18»- water - acetonitrile (40:60), column sorbent LiChrospher RP - 20 mM
KH2PO4 solution (pH 5.3) - acetonitrile (65:35). A technique to identify and quantify flutamide, and its precursor the synthesis of two metabolites in biological fluids by applying reverse phase chromatography methods.