Определение олеаноловой и глицирризиновой кислот методом тонкослойной хроматографии на обращенной фазе в водно-органических и модифицированных мицеллярных подвижных фазах
Ключевые слова:
тонкослойная хроматография, сапонины, поверхностно-активные вещества, мицеллы, спирты.
Аннотация
Методом обращенно-фазовой тонкослойной хроматографии изучено удерживание и
разделение сапонинов: олеаноловой кислоты, сапонина, глицирризиновой кислоты и хедеракозида в
водно-спиртовых и модифицированных водно-мицеллярных подвижных фазах при варьировании
концентрации поверхностно-активного вещества, природы и концентрации спирта как модификатора
мицеллярных подвижных фаз. Найдены оптимальные условия разделения указанных сапонинов и
разработаны методики определения олеаноловой и глицирризиновой кислот в растительных
фармацевтических препаратах и пищевых продуктах.
Скачивания
Данные скачивания пока не доступны.
Литература
1.Himicheskij analiz rastenij [Chemical
analysis of plants, Pod red. N.I. Grinkevich,
L.N., Safronich. M.: Vysshaja shkola, 1983, 176
p.
2.Maksjutina N.P., Komissarenko N.F.,
Prokopenko A.P. et al. Rastitel'nye
lekarstvennye sredstva [Herbal remedies], Kiev:
Zdorov'e, 1985, 280 p.
3.Murav'eva D.A., Samylina I.A., Jakovlev
G.P. Farmakognozija [Pharmacog-nosy], M.:
Medicina, 2002, 656 p.
4.Avula B., Wang Y-H., Rumulla Ch. S. et al.
Analytical methods for determination of
magnoflorine and saponins from roots of
Caulophyllum thalictroides (L.) Michx. using
UPLC, HPLC and HPTLC, J. Pharm. Biomed.
Anal., 2011, V. 56, pp. 895-903.
5.Ganzera M., Gampenrieder J., Pawar R.S. et
al. Separation of the major triterpenoids
saponins in Bacopa monnieri by highperformance
liquid chromato-graphy, Anal.
Chim. Acta, 2004, V. 516, pp. 149-154.
6.Tihomirova K.S., Borisenko R.N., Vetrova
E.V. et al. Jekstrakcija glicirrizinovoj kisloty iz
kornja solodki v srede subkriticheskoj vody
[Extraction of glycyrrhizic acid from a
glycyrrhiza root in the environment of subcritical water], Sverhkriticheskie fljuidy:
teorija i praktika, 2008, V. 3, No. 3, pp. 71-74.
7.Oleszek W., Bialy Z. Chromatographic
determination of plant saponins – An update
(2002-2005), J. Chromatogr. A, 2006, V. 1112,
pp. 78-91.
8.Gavrilin M.V., Senchenko S.P., Tamirjan
A.M., Pechenova A.V. Sover-shenstvovanie
sposobov otcenki kachestva kornej i siropa
solodki [Improvement of ways of an assessment
of quality of roots and syrup of a glycyrrhiza],
Himija rastit. syr'ja, 2009, No. 4, pp. 147-150.
9.Wang Y., Xu R., Xiao J. et al. Quanti-tative
analysis of flavonoids, alkaloids and saponins of
Banxia Xiexin decoction using ultra-high
performance liquid chromatography coupled
with electrospray ionization tandem massspectrometry,
J. Pharm. Biomed. Anal., 2014, V.
88, pp. 525-535.
10. Stavrianidi A.N., Rodin I.A., Braun A.V.
et al. Opredelenie biomarkerov P. Quinquefolius
v rastitel'nyh materialah i kommercheskih
produktah kombiniro-vannym metodom
vysokojeffektivnoj zhid-kostnoj khromatografii i
tandemnoj mass-spektrometrii [Determination of
biomarkers of P. Quinquefolius in plant
materials and commercial products by the
combined method of a high performance liquid
chromatography and tandem mass
spectrometry], Mass-spektrometrija, 2014, V.
11, No. 1, pp. 45-52.
11. Mironenko N.V., Brezhneva T.A.,
Selemenev V.F. Opredelenie triterpenovyh
saponinov - proizvodnyh oleanolovoj kisloty
metodom tonkoslojnoj hromatografii [TLC
Determination of Triterpene Saponins – the
Derivatives of Oleanolic Acid], Zavodsk. lab.
Diagnostika mater., 2011, V. 77, No. 3, pp. 22-
25.
12. Egorov M.V., Kurkin V.A., Zapesochnaja
G.G., Bykov V.A. Kachestvennyj i
kolichestvennyj analiz syr'ja i preparatov
solodki [The qualitative and quantitative
analysis of glycyrrhiza drugs and preparations],
Vestn. Voronezh. gos. un-ta. Serija: Himija.
Biologija. Farmacija, 2005, No. 1, pp. 175-180.
13. Sumina E.G., Shtykov S.N., Tyurina N.V.
Tonkoslojnaja hromatografija. Teoreticheskie
osnovy i prakticheskoe primenenie [Thin layer
chromatography. Theoretical bases and practical
application], Saratov: Izd-vo Sarat. un-ta, 2006,
112 p.
14. Fogt V.P., Stepanov A.S., Stepanova T.A.
Ispol'zovanie metodov VJeTSH i VJeZhH v
razrabotke jekstrakta protivodiabeticheskogo
[Use of methods HPTLC and HPLC in
development of antidiabetic extract], Himija
rast. syr'ja, 2008, No. 4, pp. 75-77.
15. Sumina E.G., Uglanova V.Z., Sorokina
O.N., Afonina D.O. Opredelenie stepeni chistoty
preparatov kortikoste-roidnyh gormonov
metodom tonkoslojnoj khromatografii v
podvizhnyh fazah na osno-ve ciklodekstrinov i
PAV [Determination the Degree of Purity of
Corticosteroid Hormones Preparations by Thin-
Layer Chromatography with Mobile Phases on
the Basis of Cyclodextrins and Surfactants], Izv.
Sarat. un-ta. novaja serija. Serija: Himija.
Biologija. Jekologija, 2011, V. 2, No. 2, pp.48-
54.
16. Jorshik V.M., Zhebentjaev A.I.
Issledovanie hromatograficheskogo povedenija
lekarstvennyh veshhestv antiaritmicheskogo
dejstvija metodom TSH [Investigation of
chromatographic behavior of medicinal
substances of antiarrhytmic action by TLC
method], Vestnik farmacii, 2006, No. 2-32, pp.
34-41.
17. Sumina E.G., Atajan V.Z., Shtykov S.N.
Primenenie ciklodekstrinovyh podvizhnyh faz v
tonkoslojnoj hromatografii organicheskih
reagentov ksantenovyh i hinolinovyh rjadov
[Application of cyclodextrin mobile phases in
thin layer chromatography of organic reagents
of xantene and quinoline series], Sorbtsionnye i
khromatograficheskie protsessy, 2008, V. 8, No.
1, pp. 83-93.
18. Shatc V.D., Sahartova O.V.
Vysokojeffektivnaja zhidkostnaja khromatografia
[High performance liquid chromatography],
Riga: Zinatne, 1988, 390 p.
19. Kiselev A.V. Mezhmolekuljarnye
vzaimodejstvija v adsorbcii i hromatografii
[Intermolecular Interactions in Adsorption and
Chromatography], M.: Vysshaja shkola, 1986,
360 p.
20. Berthod A., Garcia-Alvarez-Coque M.C.
Micellar Liquid Chromatography, Dekker, 2000,
632 p.
21. Basova E.M., Ivanov V.M., Shpigun O.A.
Micelljarnaja zhidkostnaja hromatografija
[Micellar liquid chroma-tography], Usp. khimii,
1999, V. 68, No. 12, pp. 1083-1101.
22. Kulikov A.Yu., Loginova L.P., Samohina
L.V. Micelljarnaja zhidkostnaja hromatografija
v farmacevticheskom analize [Micellar liquid
chromatography in pharmaceutical analysis],
Farmakom, 2004, V. 4, No. 1, pp. 22-52.
23. Armstrong D.W., Terril R.Q. Thin Layer
Chromatography Separation of Pesticides,
Decachlorobiphenyl and Nucleo-sides with
Micellar Solution, Anal. Chem., 1979, V. 51,
No. 13, pp. 2160-2164.
24. Armstrong D.W., Stine G.Y. Evaluation
of partition coefficients to micelles and
cyclodextrins via planar chromatography, J.
Amer. Chem. Soc., 1983, V. 105, No. 10, pp.
2962-2964.
25. Shtykov S.N, Sumina E.G., Smushkina
E.V., Tyurina N.V. Thin layer chromatography
of fluoresceine derivatives on direct and
reversed stationary phases with aqueous micellar
solutions, J. Planar Chromatogr. - Modern TLC,
1999, V. 12, No. 2, pp. 129-134.
26. Shtykov S.N, Sumina E.G., Tyurina N.V.
Micellar mobile phases in TLС separation of
some transition metal ions and their 1,3-
diketonates, J. Planar Chromatogr. – Modern
TLC, 2000, V. 13, No. 4, pp. 264-268.
27. Shtykov S.N., Sumina E.G., Parshina
E.V., Lopukhova S.S. Primenenie micelljarnyh
podvizhnyh faz dlja razdelenija proizvodnyh
fluoresceina metodom TSH [Use of Micellar
Mobile Phases for Separating Fluorescein
Derivatives by Means of Thin Layer
Chromatography], Zhurn. analit. khimii, 1995,
V. 50, No. 7, pp. 747-751.
28. Shtykov S.N., Sumina E.G., Smushkina
E.V., Tyurina N.V. Dynamic and Static
Modification of Stationary Phases with
Surfactants in TLC: a Comparative Study, J.
Planar Chromatogr. – Modern TLC, 2000, V.
13, No. 3, pp. 182-186.
29. Sumina E.G., Shtykov S.N., Tyurina N.V.
Poverhnostno-aktivnye veshhestva v
tonkoslojnoj hromatografii [Surfactants in Thin-
Layer Chromatography], Zhurn. analit. Himii,
2003, V. 58, No. 8, pp. 808-818.
30. Armstrong D.W., Nome F. Partitioning
behavior of solutes eluted with micellar mobile
phases in liquid chromatography, Anal. Chem.,
1981, V. 53, No. 14, pp. 1662-1666.
analysis of plants, Pod red. N.I. Grinkevich,
L.N., Safronich. M.: Vysshaja shkola, 1983, 176
p.
2.Maksjutina N.P., Komissarenko N.F.,
Prokopenko A.P. et al. Rastitel'nye
lekarstvennye sredstva [Herbal remedies], Kiev:
Zdorov'e, 1985, 280 p.
3.Murav'eva D.A., Samylina I.A., Jakovlev
G.P. Farmakognozija [Pharmacog-nosy], M.:
Medicina, 2002, 656 p.
4.Avula B., Wang Y-H., Rumulla Ch. S. et al.
Analytical methods for determination of
magnoflorine and saponins from roots of
Caulophyllum thalictroides (L.) Michx. using
UPLC, HPLC and HPTLC, J. Pharm. Biomed.
Anal., 2011, V. 56, pp. 895-903.
5.Ganzera M., Gampenrieder J., Pawar R.S. et
al. Separation of the major triterpenoids
saponins in Bacopa monnieri by highperformance
liquid chromato-graphy, Anal.
Chim. Acta, 2004, V. 516, pp. 149-154.
6.Tihomirova K.S., Borisenko R.N., Vetrova
E.V. et al. Jekstrakcija glicirrizinovoj kisloty iz
kornja solodki v srede subkriticheskoj vody
[Extraction of glycyrrhizic acid from a
glycyrrhiza root in the environment of subcritical water], Sverhkriticheskie fljuidy:
teorija i praktika, 2008, V. 3, No. 3, pp. 71-74.
7.Oleszek W., Bialy Z. Chromatographic
determination of plant saponins – An update
(2002-2005), J. Chromatogr. A, 2006, V. 1112,
pp. 78-91.
8.Gavrilin M.V., Senchenko S.P., Tamirjan
A.M., Pechenova A.V. Sover-shenstvovanie
sposobov otcenki kachestva kornej i siropa
solodki [Improvement of ways of an assessment
of quality of roots and syrup of a glycyrrhiza],
Himija rastit. syr'ja, 2009, No. 4, pp. 147-150.
9.Wang Y., Xu R., Xiao J. et al. Quanti-tative
analysis of flavonoids, alkaloids and saponins of
Banxia Xiexin decoction using ultra-high
performance liquid chromatography coupled
with electrospray ionization tandem massspectrometry,
J. Pharm. Biomed. Anal., 2014, V.
88, pp. 525-535.
10. Stavrianidi A.N., Rodin I.A., Braun A.V.
et al. Opredelenie biomarkerov P. Quinquefolius
v rastitel'nyh materialah i kommercheskih
produktah kombiniro-vannym metodom
vysokojeffektivnoj zhid-kostnoj khromatografii i
tandemnoj mass-spektrometrii [Determination of
biomarkers of P. Quinquefolius in plant
materials and commercial products by the
combined method of a high performance liquid
chromatography and tandem mass
spectrometry], Mass-spektrometrija, 2014, V.
11, No. 1, pp. 45-52.
11. Mironenko N.V., Brezhneva T.A.,
Selemenev V.F. Opredelenie triterpenovyh
saponinov - proizvodnyh oleanolovoj kisloty
metodom tonkoslojnoj hromatografii [TLC
Determination of Triterpene Saponins – the
Derivatives of Oleanolic Acid], Zavodsk. lab.
Diagnostika mater., 2011, V. 77, No. 3, pp. 22-
25.
12. Egorov M.V., Kurkin V.A., Zapesochnaja
G.G., Bykov V.A. Kachestvennyj i
kolichestvennyj analiz syr'ja i preparatov
solodki [The qualitative and quantitative
analysis of glycyrrhiza drugs and preparations],
Vestn. Voronezh. gos. un-ta. Serija: Himija.
Biologija. Farmacija, 2005, No. 1, pp. 175-180.
13. Sumina E.G., Shtykov S.N., Tyurina N.V.
Tonkoslojnaja hromatografija. Teoreticheskie
osnovy i prakticheskoe primenenie [Thin layer
chromatography. Theoretical bases and practical
application], Saratov: Izd-vo Sarat. un-ta, 2006,
112 p.
14. Fogt V.P., Stepanov A.S., Stepanova T.A.
Ispol'zovanie metodov VJeTSH i VJeZhH v
razrabotke jekstrakta protivodiabeticheskogo
[Use of methods HPTLC and HPLC in
development of antidiabetic extract], Himija
rast. syr'ja, 2008, No. 4, pp. 75-77.
15. Sumina E.G., Uglanova V.Z., Sorokina
O.N., Afonina D.O. Opredelenie stepeni chistoty
preparatov kortikoste-roidnyh gormonov
metodom tonkoslojnoj khromatografii v
podvizhnyh fazah na osno-ve ciklodekstrinov i
PAV [Determination the Degree of Purity of
Corticosteroid Hormones Preparations by Thin-
Layer Chromatography with Mobile Phases on
the Basis of Cyclodextrins and Surfactants], Izv.
Sarat. un-ta. novaja serija. Serija: Himija.
Biologija. Jekologija, 2011, V. 2, No. 2, pp.48-
54.
16. Jorshik V.M., Zhebentjaev A.I.
Issledovanie hromatograficheskogo povedenija
lekarstvennyh veshhestv antiaritmicheskogo
dejstvija metodom TSH [Investigation of
chromatographic behavior of medicinal
substances of antiarrhytmic action by TLC
method], Vestnik farmacii, 2006, No. 2-32, pp.
34-41.
17. Sumina E.G., Atajan V.Z., Shtykov S.N.
Primenenie ciklodekstrinovyh podvizhnyh faz v
tonkoslojnoj hromatografii organicheskih
reagentov ksantenovyh i hinolinovyh rjadov
[Application of cyclodextrin mobile phases in
thin layer chromatography of organic reagents
of xantene and quinoline series], Sorbtsionnye i
khromatograficheskie protsessy, 2008, V. 8, No.
1, pp. 83-93.
18. Shatc V.D., Sahartova O.V.
Vysokojeffektivnaja zhidkostnaja khromatografia
[High performance liquid chromatography],
Riga: Zinatne, 1988, 390 p.
19. Kiselev A.V. Mezhmolekuljarnye
vzaimodejstvija v adsorbcii i hromatografii
[Intermolecular Interactions in Adsorption and
Chromatography], M.: Vysshaja shkola, 1986,
360 p.
20. Berthod A., Garcia-Alvarez-Coque M.C.
Micellar Liquid Chromatography, Dekker, 2000,
632 p.
21. Basova E.M., Ivanov V.M., Shpigun O.A.
Micelljarnaja zhidkostnaja hromatografija
[Micellar liquid chroma-tography], Usp. khimii,
1999, V. 68, No. 12, pp. 1083-1101.
22. Kulikov A.Yu., Loginova L.P., Samohina
L.V. Micelljarnaja zhidkostnaja hromatografija
v farmacevticheskom analize [Micellar liquid
chromatography in pharmaceutical analysis],
Farmakom, 2004, V. 4, No. 1, pp. 22-52.
23. Armstrong D.W., Terril R.Q. Thin Layer
Chromatography Separation of Pesticides,
Decachlorobiphenyl and Nucleo-sides with
Micellar Solution, Anal. Chem., 1979, V. 51,
No. 13, pp. 2160-2164.
24. Armstrong D.W., Stine G.Y. Evaluation
of partition coefficients to micelles and
cyclodextrins via planar chromatography, J.
Amer. Chem. Soc., 1983, V. 105, No. 10, pp.
2962-2964.
25. Shtykov S.N, Sumina E.G., Smushkina
E.V., Tyurina N.V. Thin layer chromatography
of fluoresceine derivatives on direct and
reversed stationary phases with aqueous micellar
solutions, J. Planar Chromatogr. - Modern TLC,
1999, V. 12, No. 2, pp. 129-134.
26. Shtykov S.N, Sumina E.G., Tyurina N.V.
Micellar mobile phases in TLС separation of
some transition metal ions and their 1,3-
diketonates, J. Planar Chromatogr. – Modern
TLC, 2000, V. 13, No. 4, pp. 264-268.
27. Shtykov S.N., Sumina E.G., Parshina
E.V., Lopukhova S.S. Primenenie micelljarnyh
podvizhnyh faz dlja razdelenija proizvodnyh
fluoresceina metodom TSH [Use of Micellar
Mobile Phases for Separating Fluorescein
Derivatives by Means of Thin Layer
Chromatography], Zhurn. analit. khimii, 1995,
V. 50, No. 7, pp. 747-751.
28. Shtykov S.N., Sumina E.G., Smushkina
E.V., Tyurina N.V. Dynamic and Static
Modification of Stationary Phases with
Surfactants in TLC: a Comparative Study, J.
Planar Chromatogr. – Modern TLC, 2000, V.
13, No. 3, pp. 182-186.
29. Sumina E.G., Shtykov S.N., Tyurina N.V.
Poverhnostno-aktivnye veshhestva v
tonkoslojnoj hromatografii [Surfactants in Thin-
Layer Chromatography], Zhurn. analit. Himii,
2003, V. 58, No. 8, pp. 808-818.
30. Armstrong D.W., Nome F. Partitioning
behavior of solutes eluted with micellar mobile
phases in liquid chromatography, Anal. Chem.,
1981, V. 53, No. 14, pp. 1662-1666.
Опубликован
2019-11-20
Как цитировать
Sumina, E. G., Shtykov, S. N., Pankratov, A. N., Uglanova, V. Z., Tsymbal, O. A., & Danchuk, A. I. (2019). Определение олеаноловой и глицирризиновой кислот методом тонкослойной хроматографии на обращенной фазе в водно-органических и модифицированных мицеллярных подвижных фазах. Сорбционные и хроматографические процессы, 14(6). извлечено от https://journals.vsu.ru/sorpchrom/article/view/1583
Раздел
Статьи
