Выделение ферментов глиоксилатного цикла хроматографическими методами
Ключевые слова:
глиоксилатный цикл, фермент, гель-хроматография, ионообменной хроматография, очистка.
Аннотация
В статье представлен обзор по способам изоляции ключевых ферментов глиоксилатного
цикла из организмов различных таксономических групп с использованием гель-хроматографии и
ионообменной хроматографии, а также схема выделения изоцитратлиазы (ИЦЛ), малатдегидрогеназы
(МДГ) и аконитатгидратазы (АГ), предложенная исследователями кафедры биохимии и физиологии
клетки ВГУ. На первом этапе, чаще всего, используется гель-фильтрация на сефадексе с
малоразмерными порами для освобождения от низкомолекулярных примесей, затем приводится
ионообменная хроматография на диэтиламиноэтилцеллюлозе либо DEAE-Toyopearl.
Заключительным этапом является проведение гель-хроматографии с крупными размерами пор.
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Литература
1. Kornberg H.L., Krebs H. A. Synthesis of
cell constituents from C2-units by a modified
tricarboxylic acid cycle, Nature, 1957, V. 179,
pp. 988-991.
2. Eprintsev A.T., Popov V.N., Shevchenko
M.Yu. Glioksilatnyi tsikl: universal'nyi
mekhanizm adaptatsii? [Glyoxylate cycle: a
universal mechanism for adaptation], Moskva.
IKTs «Akademkniga», 2007, 228 p.
3. Eprintsev A.T., Shevchenko M.Yu., Popov
V.N. Rasprostranenie glioksilatnogo tsikla u
organizmov razlichnykh taksonomicheskikh
grupp [Distribution of the glyoxylate cycle in
organisms of different taxonomic groups] //
Uspekhi sovremennoi biologii. M., 2008, V.
128, No 3, pp. 271-280.
4. Popov V.N. , Moskalev E.A., Shevchenko
M.Yu., Eprintsev A.T. Sravnitel'nyi analiz
klyuchevogo fermenta glioksilatnogo tsikla,
izotsitratliazy, iz organizmov raznykh
sistematicheskikh grupp [Comparative analysis
of the key enzyme of the glyoxylate cycle,
isocitrate lyase, from organisms of different
taxonomic groups], Zhurnal evolyutsionnoi
fiziologii i biokhimii, 2005, No 6, pp. 776-781.
5. Determan G. Gel'-khromatografiya [Gel
chromatography], M. Mir, 1970, 173 p.
6. De Lucasa J.R., Amora C., Díazla M.,
Turnerb G., Laborda F. Purification and
properties of isocitrate lyase from Aspergillus
nidulans, a model enzyme to study catabolite
inactivation in filamentous fungi, Mycological
Research, 1997, V. 101,. No 4, pp. 410-414.
7. Eprintsev A.T., Semenova E.V., Popov
V.N. Induction of aconitate hydratase in
hepatocytes of starving rats, Biochemistry
(Mosc), 2002, V. 67, No 7, pp. 795-801.
8. Eprintsev A.T., Shevchenko M.Y., Popov
V.N. Purification and properties of isocitrate
lyase from pupas of the butterfly Papilio
machaon L, Biochemistry (Mosc), 2004, V. 69,
No 4, pp. 376-80.
9. Hoyt J.C., Johnson K.E., Reeves H.C.
Purification and characterization of
Acinetobacter calcoaceticus isocitrate lyase, J
Bacteriol, 1991, V. 173, No 21, pp. 6844-6848.
10. John P.C., Syrett P.J. The purification and
properties of isocitrate lyase from Chlorella,
Biochem. J, 1967, V. 105, No 1, pp. 409-416.
11. Kamel M.Y., Fahmy A.S. Biochemical
studies of tick embriogenesis. Purification and
partial characterisation of isocitrate lyase from
eggs of the tick Hyalomma dromedarii,Comp.
Biochem. Physiol. B, 1982, V. 72, pp. 107-115.
12. Liu F., Thatcher J.D., Barral J.M.
Bifunctional glyoxylate cycle protein of
Caenorhabditis elegans: a developmentally
regulated protein of intestine and muscle, Dev.
Biol, 1995, V. 169, pp. 399-414.
13. Lopez-Boado Y.S., Herrero P., Fernandez
M.T., Fernandez R., Moreno F. Purification of
isocitrate lyase from Saccharomyces cerevisiae,
Yeast, 1988, V. 4, No 1,pp. 41-46.
14. Munir E., Hattori T., Shimada M.
Purification and characterization of isocitrate
lyase from the wood-destroying basidiomycete
Fomitopsis palustris grown on glucose, Arch.
Biochem. Biophys, 2002, V. 399, No 2, pp.
225-231.
15. Nakamura K., Amano Y., Nakadate M.,
Kagami M. Purification and properties of
isocitrate lyase from candida brassicae e 17. J.
of Fermentation & Bioengineering, 1989, V.
67, No 3, pp. 153-157.
16. Popov V.N., Igamberdiev A.U.,
Schnarrenberger C., Volvenkin S.V. Induction
of glyoxylate cycle enzymes in rat liver upon
food starvation, FEBS Lett, 1996, V. 390, No 3.
pp. 258-260.
17. Popov V.N., Volvenkin S.V., Eprintsev
A.T., Igamberdiev A.U. Induction of glyoxylate
cycle enzymes in various tissues from starving
rats, Izv. Akad Nauk Ser. Biol, 2000, V. 6, pp.
672-678.
18. Popov V.N., Volvenkin S.V., Eprintsev
A.T., Igamberdiev A.U. Glyoxylate cycle
enzymes are present in liver peroxisomes of
alloxan-treated rats, FEBS Lett, 1998, V. 440,
No 1-2, pp. 55-58.
19. Popov V.N., Volvenkin S. V., Kosmatykh
T. A. et al. Induction of a peroxisomal malate
dehydrogenase isoform in liver of starved rats,
Biochemistry (Mosc), 2001, V. 66, No 5, pp.
496-501.
20. Ruchti M., Widmer F. Isocitrate lyase
from germinating soybean cotyledons:
purification and characterization, J. exp. Bot,
1986, V. 37, pp. 1685-1690.
21. Vincenzini M.T., Nerozzi F., Vincieri
F.F., Vanni P. Isolation and properties of
isocitrate lyase from Lupinus seed,
Phytochemistry, 1980, V. 19, No 5, pp. 769-
774.
22. Watanabe S., Yamaoka N., Fukunaga N.,
Takada Y. Purification and characterization of a
cold-adapted isocitrate lyase and expression
analysis of the cold-inducible isocitrate lyase
gene from the psychrophilic bacterium
Colwellia psychrerythraea, Extremophiles,
2002, V. 6, No 5, pp. 397-405.
23. Dingman D.W., Sonenshein A.L.
Purification of aconitase from Bacillus subtilis
and correlation of its N-terminal amino acid
sequence with the sequence of the citB gene, J.
Bacteriol, 1987, V. 169, No 7, pp. 3062-3067.
cell constituents from C2-units by a modified
tricarboxylic acid cycle, Nature, 1957, V. 179,
pp. 988-991.
2. Eprintsev A.T., Popov V.N., Shevchenko
M.Yu. Glioksilatnyi tsikl: universal'nyi
mekhanizm adaptatsii? [Glyoxylate cycle: a
universal mechanism for adaptation], Moskva.
IKTs «Akademkniga», 2007, 228 p.
3. Eprintsev A.T., Shevchenko M.Yu., Popov
V.N. Rasprostranenie glioksilatnogo tsikla u
organizmov razlichnykh taksonomicheskikh
grupp [Distribution of the glyoxylate cycle in
organisms of different taxonomic groups] //
Uspekhi sovremennoi biologii. M., 2008, V.
128, No 3, pp. 271-280.
4. Popov V.N. , Moskalev E.A., Shevchenko
M.Yu., Eprintsev A.T. Sravnitel'nyi analiz
klyuchevogo fermenta glioksilatnogo tsikla,
izotsitratliazy, iz organizmov raznykh
sistematicheskikh grupp [Comparative analysis
of the key enzyme of the glyoxylate cycle,
isocitrate lyase, from organisms of different
taxonomic groups], Zhurnal evolyutsionnoi
fiziologii i biokhimii, 2005, No 6, pp. 776-781.
5. Determan G. Gel'-khromatografiya [Gel
chromatography], M. Mir, 1970, 173 p.
6. De Lucasa J.R., Amora C., Díazla M.,
Turnerb G., Laborda F. Purification and
properties of isocitrate lyase from Aspergillus
nidulans, a model enzyme to study catabolite
inactivation in filamentous fungi, Mycological
Research, 1997, V. 101,. No 4, pp. 410-414.
7. Eprintsev A.T., Semenova E.V., Popov
V.N. Induction of aconitate hydratase in
hepatocytes of starving rats, Biochemistry
(Mosc), 2002, V. 67, No 7, pp. 795-801.
8. Eprintsev A.T., Shevchenko M.Y., Popov
V.N. Purification and properties of isocitrate
lyase from pupas of the butterfly Papilio
machaon L, Biochemistry (Mosc), 2004, V. 69,
No 4, pp. 376-80.
9. Hoyt J.C., Johnson K.E., Reeves H.C.
Purification and characterization of
Acinetobacter calcoaceticus isocitrate lyase, J
Bacteriol, 1991, V. 173, No 21, pp. 6844-6848.
10. John P.C., Syrett P.J. The purification and
properties of isocitrate lyase from Chlorella,
Biochem. J, 1967, V. 105, No 1, pp. 409-416.
11. Kamel M.Y., Fahmy A.S. Biochemical
studies of tick embriogenesis. Purification and
partial characterisation of isocitrate lyase from
eggs of the tick Hyalomma dromedarii,Comp.
Biochem. Physiol. B, 1982, V. 72, pp. 107-115.
12. Liu F., Thatcher J.D., Barral J.M.
Bifunctional glyoxylate cycle protein of
Caenorhabditis elegans: a developmentally
regulated protein of intestine and muscle, Dev.
Biol, 1995, V. 169, pp. 399-414.
13. Lopez-Boado Y.S., Herrero P., Fernandez
M.T., Fernandez R., Moreno F. Purification of
isocitrate lyase from Saccharomyces cerevisiae,
Yeast, 1988, V. 4, No 1,pp. 41-46.
14. Munir E., Hattori T., Shimada M.
Purification and characterization of isocitrate
lyase from the wood-destroying basidiomycete
Fomitopsis palustris grown on glucose, Arch.
Biochem. Biophys, 2002, V. 399, No 2, pp.
225-231.
15. Nakamura K., Amano Y., Nakadate M.,
Kagami M. Purification and properties of
isocitrate lyase from candida brassicae e 17. J.
of Fermentation & Bioengineering, 1989, V.
67, No 3, pp. 153-157.
16. Popov V.N., Igamberdiev A.U.,
Schnarrenberger C., Volvenkin S.V. Induction
of glyoxylate cycle enzymes in rat liver upon
food starvation, FEBS Lett, 1996, V. 390, No 3.
pp. 258-260.
17. Popov V.N., Volvenkin S.V., Eprintsev
A.T., Igamberdiev A.U. Induction of glyoxylate
cycle enzymes in various tissues from starving
rats, Izv. Akad Nauk Ser. Biol, 2000, V. 6, pp.
672-678.
18. Popov V.N., Volvenkin S.V., Eprintsev
A.T., Igamberdiev A.U. Glyoxylate cycle
enzymes are present in liver peroxisomes of
alloxan-treated rats, FEBS Lett, 1998, V. 440,
No 1-2, pp. 55-58.
19. Popov V.N., Volvenkin S. V., Kosmatykh
T. A. et al. Induction of a peroxisomal malate
dehydrogenase isoform in liver of starved rats,
Biochemistry (Mosc), 2001, V. 66, No 5, pp.
496-501.
20. Ruchti M., Widmer F. Isocitrate lyase
from germinating soybean cotyledons:
purification and characterization, J. exp. Bot,
1986, V. 37, pp. 1685-1690.
21. Vincenzini M.T., Nerozzi F., Vincieri
F.F., Vanni P. Isolation and properties of
isocitrate lyase from Lupinus seed,
Phytochemistry, 1980, V. 19, No 5, pp. 769-
774.
22. Watanabe S., Yamaoka N., Fukunaga N.,
Takada Y. Purification and characterization of a
cold-adapted isocitrate lyase and expression
analysis of the cold-inducible isocitrate lyase
gene from the psychrophilic bacterium
Colwellia psychrerythraea, Extremophiles,
2002, V. 6, No 5, pp. 397-405.
23. Dingman D.W., Sonenshein A.L.
Purification of aconitase from Bacillus subtilis
and correlation of its N-terminal amino acid
sequence with the sequence of the citB gene, J.
Bacteriol, 1987, V. 169, No 7, pp. 3062-3067.
Опубликован
2019-11-20
Как цитировать
Syromyatnikov, M. Y., Eprintsev, A. T., & Popov, V. N. (2019). Выделение ферментов глиоксилатного цикла хроматографическими методами. Сорбционные и хроматографические процессы, 14(6). извлечено от https://journals.vsu.ru/sorpchrom/article/view/1584
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