The application of CAMAG TLC-MS Interface in two-dimensional TLC: TLC – capillary GC

Abstract

The combined consecutive application of several chromatographic methods (for example, TLC and GC) for
identification of components of the researched mixtures is, undoubtedly, of interest, since it increases its informativity
and reliability. When using TLC as a method of two-dimensional chromatography, the main problem is the transfer of
separated zones of compounds with TLC plate to column chromatography for further separation. To solve this problem,
recently developed the device "CAMAG TLC-MS Interface" (CAMAG) has been applied for the extraction of the zones
from the TLC plate when using the methods of both circular and two-dimensional elution within identifying the
qualitative composition of the individual chromatographic zones on the TLC plates. As objects of the investigation the
mixtures of steroids and chlorophenols were used. The application of CAMAG TLC-MS Interface in n-dimensional
chromatography allowed rapidly and reliably identifying qualitatively separated compounds (what is especially actual
when analyzing the unknown samples). For the first time, it is shown that the device CAMAG TLC-MS Interface can be
used for the different variants of circular (for example, of corner and lateral TLC), two-dimensional TLC and other
variants of TLC