Kinetic characteristics of tetrameric malate dehydrogenase from animals and bacteria, produced by ion-exchange chromatography

Authors

  • Abud Faris Satar graduate student, Department of Biochemistry and Cell Physiology Voronezh State University, Voronezh
  • Irina V. Parfenova graduate student, Department of Biochemistry and Cell Physiology Voronezh State University, Voronezh
  • Elena V. Maltseva graduate student, Department of Biochemistry and Cell Physiology Voronezh State University, Voronezh
  • Marina I. Falaleeva Candidate of Biology, Department of Biochemistry and Cell Physiology Voronezh State University, Voronezh
  • Alexander T. Eprintsev Doctor of Biology, Department of Biochemistry and Cell Physiology Voronezh State University, Voronezh

Keywords:

malate dehydrogenase (MDH), isoforms, dimer, tetramer, kinetic properties.

Abstract

Homogeneous isozymes of malate dehydrogenase (MDH) were purified from bacteria
Sphaerotilus natans D-507 (with specific activity of 2,78 and 3,37 units/mg protein) and rat liver (with
specific activity of 2,19 and 1,77 units/mg protein). Presence of dimeric and tetrameric MDH were
determined. Isoforms were separated by ion-exchange chromatography. Kinetic characteristics of
tetrameric enzyme were studied. Organism response on changing conditions connects with new isoform
formation

Downloads

Download data is not yet available.

References

Downloads

Published

2019-11-27

Issue

Vol. 10 No. 2 (2010): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

Kinetic characteristics of tetrameric malate dehydrogenase from animals and bacteria, produced by ion-exchange chromatography. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 10(2). https://journals.vsu.ru/sorpchrom/article/view/2042

Most read articles by the same author(s)