HPLC Study of Humus Acids Isolated from Composts and Vermicomposts of Different Period of Matured

Authors

  • Ninel E. Pavlovskaya professor, head of the department of Biotechnology, Orel State Agriculture University
  • Elena I. Yushkova cand. Chem. Sci., the senior lecturer faculty of Pharmacology and Biochemistry of Medical Institute, Orel State University, tel. (462)432181, E-mail: kin1@orel.ru
  • Anatoliy N. Danilenko senior research worker of N. M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, E-mail: adanilenko@yandex.ru

Keywords:

humus asids, vermicompost, HPLC, factional composition, relatively aromaticity.

Abstract

Humic acids (HA) isolated from the horse composts and vermicomposts of different period of
matured were studied by high-performance liquid chromatography (HPLC) method. All chromatograms
contained three peaks. HA corresponding to the first and the second peaks were hydrophilic and were
eluted from the column with water solution of phosphate buffer, whereas HA, which corresponded to the
third peak, were hydrophobic and were eluted with acetonytril. Relative aromaticity of total factions were
lowering in series: control (0,59) > winter-sample (0,49) > summer-sample (0,33), which indicated the
greater degree of humification of summer-sample of vermicompost.

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Published

2019-11-27

Issue

Vol. 10 No. 3 (2010): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

HPLC Study of Humus Acids Isolated from Composts and Vermicomposts of Different Period of Matured. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 10(3). https://journals.vsu.ru/sorpchrom/article/view/2061