Liquid chromatography of trombocidins
Keywords:
trombocidins, liquid chromatography, solid phase extraction, low molecular weight cationic (anionic) proteins.Abstract
Thrombocidal low molecular weight proteins (TLMWP) – trombocidins- belongs to class of
micromicidal low molecular weight proteins, produced as a result of response of immune system of
majority of live organisms. Current publication is devoted to development of novel chromatographic
procedures for separation, purification and analysis of TLMWP, isolated from For this purpose complex
stepwise chromatographical procedure for isolation and analysis of proteins was proposed, which includes
re-suspending of human packed red cells in acetic acid at 258 K with further centrifugation and
purification using solid phase extraction (SPE) procedure and high performance liquid chromatography
(HPLC) analysis. Analytical procedures (high performance thin layer chromatography (HPTLC) and
HPLC) for analysis of trombocidins were developed
