Determination of ethylenediaminetetraacetic acid in mayonnaise by ion-pair reversed phase high performance liquid chromatography

Authors

  • Anna M. Zaharova the post-graduate student of analytical chemistry department of chemical faculty, St. Petersburg state university, engineer of Analytical Laboratory Analyt Ltd St. Petersburg, St. Petersburg, e-mail: za@analitspb. ru
  • Ilia L. Grinshein Cand.Sc. Chem., head of the Analit group, member of RAS Analytical Chemistry Scientific Committee, St. Petersburg, e-mail: grin@analit-spb.ru
  • Ludmila A. Kartsova Dr.Sc.Chem. the professor of organic chemistry department of chemical faculty, St. Petersburg state university, St. Petersburg, e-mail: kartsova@gmail.com
  • Vera E. Potolitsyna the post-graduate student of analytical chemistry department of chemical faculty, St. Petersburg state university, , St. Petersburg, e-mail: potolitsynavera@gmail.com

Keywords:

ion-pair RP HPLC with spectrofotometric detection, ethylenediaminetetraacetic acid, ferric chloride (III), mayonnaise.

Abstract

Determination of ethylenediaminetetraacetic acid (EDTA) in the mayonnaise samples by ion-pair
RP HPLC with spectrophotometric detection was investigated. Extraction of EDTA was performed with
phosphate buffered solution (pH = 7.5). For spectrophotometric registration was prepared complex with
ferric chloride (III). Chromatographic separation was performed on a reverse phase column in isocratic
elution mode, in mobile phase was added to an ion-pair reagent - tetrabutylammonium hydroxide. Detection
wavelength 255 nm.

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Published

2019-11-18

Issue

Vol. 14 No. 1 (2014): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

Determination of ethylenediaminetetraacetic acid in mayonnaise by ion-pair reversed phase high performance liquid chromatography. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 14(1). https://journals.vsu.ru/sorpchrom/article/view/1437

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