Respiratory nitrate reductase from Thiothrix lacustris AS: purification, physic-chemical properties and catalytic characteristics

Authors

  • Ivan V. Trubitsin PhD student, Voronezh State University, the Biology Department, Division of Cell Biochemistry and Physiology, Voronezh; email: tru.ivan@mail.ru
  • Margarita Yu. Grabovich Doctor of Biology, professor, Voronezh State University, the Biology Department, Division of Cell Biochemistry and Physiology, Voronezh e-mail: margarita_grabov@mail.ru
  • Maria N. Tutukina PhD in Molecular biology, senior research scientist in the laboratory of Functional genomics and cellular stress, Institute of Cell Biophysics RAS, Pushchino, maria@icb.psn.ru

Keywords:

respiratory nitrate reductase, Thiothrix lacustris AS, purification.

Abstract

Respiratory nitrate reductase (E.C. 1.7.5.1) from bacterium Thiothrix lacustris AS cultivated under
anaerobic conditions in the presence of nitrate was isolated and purified 78-fold using a multistep purification
scheme including gel-filtration, ion-exchange chromatography, and preparative electrophoresis. The enzyme
was homogenous according to electrophoretic data, with activity of 97,7 U /mg. Kinetic and physicalchemical
characteristics of the enzyme (pH and temperature optima, thermostability, effect of different
inhibitors on activity) were studied.

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Published

2019-11-19

Issue

Vol. 14 No. 2 (2014): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

Respiratory nitrate reductase from Thiothrix lacustris AS: purification, physic-chemical properties and catalytic characteristics. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 14(2). https://journals.vsu.ru/sorpchrom/article/view/1479

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