Determination of anthocyanins in fruits of some species of grapes by HPLC
Keywords:
reversed phase HPLC, grape, 3,5-diglucosides, 3-glucosides, cyanidin, delphinidin, petunidin, peonidin, malvidin, separation map.Abstract
The aim of the paper was investigation of chromatographic behaviour of five major anthocyanidin 3-
glucosides and 3,5-diglucosides for working out reversed-phase HPLC method for the hybrid grape fruit skin
anthcyanins separation. As objects, some hybrid grapes grown in Belgorod were chosen.
Chromatographic behavior of the anthocyanins was analyzed in the framework of relative retention
analysis. Visible spectra, mass-spectrometric data as well as peak effectivity control were utilized for the
anthocyanin identification.
For the separation, an effective isocratic reversed-phase HPLC procedure was proposed. It was
found that in case of V. amurensis, V. flexuosa, V. labrusca and V. coignetiae species fruits and for Moldova
hybrid variety the presence of large amounts (up to 90%) of 3,5-diglucosides of all five anthocyanidines is
typical. Nevertheless for many for not winter-hardy Belgorod hybrid grape varieties the activity of 5-
glucosyltranferase is suppressed.
The method propose may be used for chemosystematics of hybrid grape varieties. However, for
some winter-hardy species biosynthesis of 3.5-diglucosides may be suppressed to produce typical for V.
vinifera anthocyanins. Thus, HPLC is a powerful approach for heredity control at plant breeding.
