Reversed-phase HPLC fir determination of chocolate cocoa butter authenticity
Keywords:
chocolate, cocoa butter, authenticity determination, HPLC, vector model.Abstract
Reversed-phase HPLC has been applied for authenticity determination of cocoa butter in
chocolate and glaze of chocolates. It has been shown that in some cases qualitative consideration of
relative areas of peaks of the three main triacylglycerols (oleate-dipalmitate, OP2; oleate-palmitatesrearate,
OPS; oleate-distearate, OS2) is just enough to solve the problem. For quantitate calculation the
vector model is proposed, - namely the angle between two vectors in the three-dimensional space based
upon mole portions of OP2, OPS and OS2 for the oil under investigation and for original cocoa butter.









