Determination of imatinibe in human plasma using HPLC with diode-array detection.
Keywords:
imatinib, plasma, HPLC.Abstract
The paper of Shohin I.E., Ramenskaya G.V., Medvedev Yu.V., Yarushok T.A. and Shamal L.L.
is devoted to development and validation of HPLC method determination of imatinib in human plasma
with diode-array detection. Sample preparation was made by proteins precipitations using acetonitrile.
HPLC analysis was performed on C18 (2) column (100 A, 5 μm, 4,6 x 250 mm). Methanol –phosphate
buffer pH 2.50 (54:46) at 1.0 mL/min was used as mobile phase. The standard curve was linear over the
range 0.40 μg/ml to 8.05 μg/ml of imatinib in plasma. Within-run precision was 3.31 % to 8.78 % and
between-run precision was 4.70 % to 8.25 % determined on spiked samples. The accuracy of the method
was – 10,07 % to 15,84 % (within-run) and 5,63 % to 11.19 (between-run). The lower limit of
quantification was 0.40 μg/ml. The method was applied to comparative pharmacokinetics study of
imatinib drug products in patients.
