The obtaining of succinate dehydrogenase from flight muscles of bumble bees Bombus terrestris using ion-exchange chromotography

Authors

  • Tatyana M. Gorbacheva graduate student, Department of biochemistry and physiology, Voronezh State University, Voronezh, E-mail: sokitanya@ yandex.ru
  • Mihail Yu. Syromyatnikov master of biology, Department of biochemistry and physiology, Voronezh State University, Voronezh
  • Vasilij N. Popov doctor of biology, professor, Department of biochemistry and physiology, Voronezh State University, Voronezh
  • Aleksej V. Lopatin candidate of biology, Department of entomology, Voronezh State University, Voronezh

Keywords:

succinate dehydrogenase (SDH), ion-exchange chromatography, isofom, bumble bee, Bombus terrestris.

Abstract

An effective procedure for purification of the succinate dehydrogenase from mitochondria of
flight muscles of bumble bee Bombus terrestris has been developed. The procedure includes fractionation
of proteins from the mitochondrial fraction by ammonium sulfate, gel filtration, ion-exchange
chromatography on DEAE-cellulose. The enzyme was purified up to homogeneity. The specific activity
of homogeneous preparation was 7.14 units per mg of protein. The enzyme from flight muscles of bumble
bee is made up of one isoform. The substrate specificity has been investigated

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Published

2019-11-26

Issue

Vol. 11 No. 5 (2011): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

The obtaining of succinate dehydrogenase from flight muscles of bumble bees Bombus terrestris using ion-exchange chromotography. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 11(5). https://journals.vsu.ru/sorpchrom/article/view/1973