Synthesis and chromatographic properties of monolithic columns based on copolymers of divinylbenzene and ethylvinylbenzene with hydroxyalkyl methacrylates
Keywords:
monolithic column, divinylbenzene, hydroxyalkyl methacrylate, HPLC.Abstract
Monolithic stationary phases based on copolymers of divinylbenzene (DVB) and
ethylvinylbenzene (EVB) with various hydroxyalkyl methacrylates were synthesized via thermally
initiated free-radical polymerization inside glass tubes with dimensions of 150 × 3 mm. The use of
hydroxybutyl methacrylate instead of hydroxyethyl methacrylate (HEMA) was found to increase the
column permeability by a factor of 1,6 with 5–10% drop in efficiency. The effect of column temperature
on separation using poly(DVB-co-EVB-co-HEMA) monolith was studied. Raising the temperature from
25°C to 80°C increased the efficiency of the column and maximum flow rate of the mobile phase and
reduced the influence of retention factor on peak broadening. At 80°C and velocities up to 3,7 mm/s the
efficiency for alkylbenzenes was 19000–20000 plates/m
