Appliance of sorbent-based extraction method to optimize rat liver tissue DNA isolation protocol

Authors

  • Victor Yu. Bashmakov postgraduate at Genetics, Cytology and Bioengineering Dep., Faculty of Biology and Soil Sciences, Voronezh State University, Voronezh, еmail: vitomail2012@ yandex.ru
  • Sergey A. Solodskikh 3rd year student at Genetics, Cytology and Bioengineering Dep., Faculty of Biology and Soil Sciences, Voronezh State University, Voronezh
  • Anna V. Panyovina 4rd year student at Genetics, Cytology and Bioengineering Dep., Faculty of Biology and Soil Sciences, Voronezh State University, Voronezh
  • Maria L. Shmatkova 4rd year student at Genetics, Cytology and Bioengineering Dep., Faculty of Biology and Soil Sciences, Voronezh State University, Voronezh
  • Vasily N. Popov professor, Dr. Biol., Head of Genetics, Cytology and Bioengineering Dep., Faculty of Biology and Soil Sciences, Voronezh State University, Voronezh

Keywords:

DNA, phenol-chlorophorm extraction, silica sorbent, electrophoresis.

Abstract

Purity of nucleic acids, the way they were obtained and nucleic acid yield are crucial for many
molecular biology techniques, studying the physicochemical properties of nucleic acids and chemical
modification. Though pnenol-chlorophorm-based extraction of DNA remains traditional, other
approaches are also widely-used, such as magnetic particles, chromatography or sorbents usage. The
appliance of two different techniques (pnenol-chlorophorm extraction and sorbent-based isolation) is
observed in the present article

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Published

2019-11-25

Issue

Vol. 12 No. 5 (2012): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

Appliance of sorbent-based extraction method to optimize rat liver tissue DNA isolation protocol. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 12(5). https://journals.vsu.ru/sorpchrom/article/view/1862

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