Use of the hyperbranched (hypercrosslinked) polystyrene as sorbent for solid-phase extraction at the analysis of drugs (medicines) in biological objects with high performance thin layer chromatography (HPTLC)

Abstract

Possibility of simultaneous determination hydrophobic and hydrophilic drugs (medical
products), used at treatment of various dysfunctions of adrenal glands, and on real objects (urine and
serum) by method HPTLC with densitometry detection with use off-line concentration on sorbent
hyperbranched polystyrene Purosep-270 and on-line on a plate was shown. The comparative quantitative
estimation liquid and solid-phase extraction on С18, silicagel and hyperbranched polystyrene Purosep-
270 have been done. The increase in selectivity of division has been developed with use of various
modifiers of a stationary phase (β-cyclodecstrene (β-CD) and cationic and anionic surface-active
substances).