TLC separation of D-, L- of amino acids in the 2-hydroxypropyl-β-cyclodextrin aqueous mobile phase

Abstract

The chromatographic separation of DL-isomers of tyrosine, leucine, tryptophan at Sorbfil TLC plates
in aqueous mobile phase modified by 2-hydroxypropyl-β-cyclodextrin has been performed. It has been
established that the modifier enhances the separation of enantiomers. A mobility of L-isomers of amino acids
increases with increasing a concentration of the cyclodextrin in the mobile phase, whereas d-isomers – is
unaffected. D- and L-isomers of the studied amino acids in Еру pharmaceutical preparations "Neframin" and
"Gidrolizin" have been analysed and L-isomers of aminoacids investigated was identified. The identification
was proved by comparison with individual aminoacids