Self-assembly of cationic polymers in mixed polymer-lipid monolayers at the liquid-air interface
Abstract
Purpose: The self-assembly of cationic polymers at the liquid-air interface is the most important process for the design of new targeted drug delivery systems, including lipid-polymer hybrid nanoparticles.
We studied the self-assembly of cationic pH-sensitive polymers at the liquid-air interface in mixed Langmuir polymer-lipid monolayers of cholesterol with Lipoid S100.
Experimental: We used synthetic approaches to the synthesis of ionene polymer and studied its physicochemical properties by NMR, IR, HPLC, and gel permeation chromatography. The surface behavior and states of polymer monolayers and their mixtures with lipids (compressibility and molecular area) at the liquid-air interface were examined using the Langmuir-Blodgett technique. The resulting lipoplexes were studied by dynamic light scattering (average weight diameter and zeta potential).
Conclusions: High stability of lipid-polymer nanoparticles was achieved for compositions of mixed monolayers with the compressibility modulus (Cs–1) of at least 50 mN/m. In this case, the particle sizes were in the range from 32 to 73 nm and the zeta potential values for non-quaternized cationic polymers were strongly negative (from –15 to –45 mV), while for the ionene polymers they were significantly positive (from +8 to +49 mV)
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