Self-assembly of cationic polymers in mixed polymer-lipid monolayers at the liquid-air interface

  • Denis M. Kamorin Nizhny Novgorod State Technical University n.a. R. E. Alekseev, 24 Minina ul., Nizhny Novgorod 603155, Russian Federation; Lobachevsky National Research State University of Nizhny Novgorod, 23 Gagarin pr., Nizhny Novgorod 603022, Russian Federation https://orcid.org/0000-0002-8712-5062
  • Alexander S. Simagin Nizhny Novgorod State Technical University n.a. R. E. Alekseev, 24 Minina ul., Nizhny Novgorod 603155, Russian Federation; Lobachevsky National Research State University of Nizhny Novgorod, 23 Gagarin pr., Nizhny Novgorod 603022, Russian Federation https://orcid.org/0000-0002-6985-2098
  • Oleg A. Kazantsev 1Nizhny Novgorod State Technical University n.a. R. E. Alekseev, 24 Minina ul., Nizhny Novgorod 603155, Russian Federation https://orcid.org/0000-0003-1536-3966
  • Aleksei I. Slivkin Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation https://orcid.org/0000-0001-6934-0837
  • Olga G. Zamyshlyayeva Nizhny Novgorod State Technical University n.a. R. E. Alekseev, 24 Minina ul., Nizhny Novgorod 603155, Russian Federation; Lobachevsky National Research State University of Nizhny Novgorod, 23 Gagarin pr., Nizhny Novgorod 603022, Russian Federation https://orcid.org/0000-0002-4331-3125
  • Anastasia A. Emasheva Lobachevsky National Research State University of Nizhny Novgorod, 23 Gagarin pr., Nizhny Novgorod 603022, Russian Federation https://orcid.org/0009-0008-6097-4831
  • Nina B. Melnikova Nizhny Novgorod State Technical University n.a. R. E. Alekseev, 24 Minina ul., Nizhny Novgorod 603155, Russian Federation; Lobachevsky National Research State University of Nizhny Novgorod, 23 Gagarin pr., Nizhny Novgorod 603022, Russian Federation https://orcid.org/0000-0003-1335-1048
Keywords: Self-assembly at interfaces, Polymer-lipid nanoparticles, Langmuir monolayers, Ionene polymers

Abstract

Purpose: The self-assembly of cationic polymers at the liquid-air interface is the most important process for the design of new targeted drug delivery systems, including lipid-polymer hybrid nanoparticles.
We studied the self-assembly of cationic pH-sensitive polymers at the liquid-air interface in mixed Langmuir polymer-lipid monolayers of cholesterol with Lipoid S100.

Experimental: We used synthetic approaches to the synthesis of ionene polymer and studied its physicochemical properties by NMR, IR, HPLC, and gel permeation chromatography. The surface behavior and states of polymer monolayers and their mixtures with lipids (compressibility and molecular area) at the liquid-air interface were examined using the Langmuir-Blodgett technique. The resulting lipoplexes were studied by dynamic light scattering (average weight diameter and zeta potential).

Conclusions: High stability of lipid-polymer nanoparticles was achieved for compositions of mixed monolayers with the compressibility modulus (Cs–1) of at least 50 mN/m. In this case, the particle sizes were in the range from 32 to 73 nm and the zeta potential values for non-quaternized cationic polymers were strongly negative (from –15 to –45 mV), while for the ionene polymers they were significantly positive (from +8 to +49 mV)

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Author Biographies

Denis M. Kamorin, Nizhny Novgorod State Technical University n.a. R. E. Alekseev, 24 Minina ul., Nizhny Novgorod 603155, Russian Federation; Lobachevsky National Research State University of Nizhny Novgorod, 23 Gagarin pr., Nizhny Novgorod 603022, Russian Federation

Cand. Sci. (Chem.), Associate Professor of the Department of Chemical and Food
Technologies, Nizhny Novgorod State Technical University
n.a. R. E. Alekseev (Nizhny Novgorod, Russian Federation)

Alexander S. Simagin, Nizhny Novgorod State Technical University n.a. R. E. Alekseev, 24 Minina ul., Nizhny Novgorod 603155, Russian Federation; Lobachevsky National Research State University of Nizhny Novgorod, 23 Gagarin pr., Nizhny Novgorod 603022, Russian Federation

Cand. Sci. (Chem.), Lecturer at the Department of Chemical and Food Technologies, Nizhny
Novgorod State Technical University n.a. R. E. Alekseev (Nizhny Novgorod, Russian Federation)

Oleg A. Kazantsev, 1Nizhny Novgorod State Technical University n.a. R. E. Alekseev, 24 Minina ul., Nizhny Novgorod 603155, Russian Federation

Dr. Sci. (Chem.), Professor, Head of the Department of Chemical and Food Technologies, Nizhny
Novgorod State Technical University n.a. R. E. Alekseev (Nizhny Novgorod, Russian Federation)

Aleksei I. Slivkin, Voronezh State University, 1 Universitetskaya pl., Voronezh 394018, Russian Federation

Dr. Sci. (Pharm.), Full Professor, Head of the Department of Pharmaceutical Technology, Voronezh
State University (Voronezh Russian Federation)

Olga G. Zamyshlyayeva, Nizhny Novgorod State Technical University n.a. R. E. Alekseev, 24 Minina ul., Nizhny Novgorod 603155, Russian Federation; Lobachevsky National Research State University of Nizhny Novgorod, 23 Gagarin pr., Nizhny Novgorod 603022, Russian Federation

Dr. Sci. (Pharm.), Professor at the Department of Macromolecular Compounds and Colloid
Chemistry, Lobachevsky State University of Nizhni Novgorod (Nizhny Novgorod, Russian Federation)

Anastasia A. Emasheva, Lobachevsky National Research State University of Nizhny Novgorod, 23 Gagarin pr., Nizhny Novgorod 603022, Russian Federation

postgraduate student, Junior Research Fellow at the Center for Molecular Biology and
Biomedicine, Lobachevsky State University of Nizhni Novgorod (Nizhny Novgorod, Russian Federation)

Nina B. Melnikova, Nizhny Novgorod State Technical University n.a. R. E. Alekseev, 24 Minina ul., Nizhny Novgorod 603155, Russian Federation; Lobachevsky National Research State University of Nizhny Novgorod, 23 Gagarin pr., Nizhny Novgorod 603022, Russian Federation

Dr. Sci. (Chem.), Professor at the Department of Analytical and Medicinal Chemistry,
Lobachevsky State University of Nizhni Novgorod (Nizhny Novgorod, Russian Federation)

References

Zhou W., Jiang L., Liao S., … Zhang Y. Vaccines’ newera-RNA vaccine. Viruses. 2023;15(8): 1760. https://doi.org/10.3390/v15081760

Wang Y., Zhang R., Tang L., Yang L. Nonviral delivery systems of mRNA vaccines for cancer gene therapy. Pharmaceutics. 2022;14(3): 512. https://doi.org/10.3390/pharmaceutics14030512

Santhanes D., Zhang H., Wilkins A., John Aitken R., Gannon A.L., Liang M. Engineering pH-sensitive dissolution of lipid-polymer nanoparticles by Eudragit integration impacts plasmid DNA (pDNA) transfection. European Journal of Pharmaceutics and Biopharmaceutics. 2024;199: 114299. https://doi.org/10.1016/j.ejpb.2024.114299

Gargouri M., Sapin A., Bouli S., Becuwe P., Merlin J. L., Maincent P. Optimization of a new non-viral vector for transfection: eudragit nanoparticles for the delivery of a DNA plasmid. Technology in Cancer Research and reatment. 2009; 8(6): 433-44. https://doi.org/10.1177/153303460900800605

Doerdelmann G., Kozlova D., Epple M. A pH-sensitive poly(methyl methacrylate) copolymer for efficient drug and gene delivery across the cell membrane. Journal of Materials Chemistry B. 2014;2(41): 7123–7131. https://doi.org/10.1039/C4TB01052C

Tsutsui T. Developments in ionic polymers. Chapter 4. A. D. Wilson, H. J. Prossser (eds.). Netherlands: Springer Dordrecht; 1986. p. 163–189. https://doi.org/10.1007/978-94-009-4187-8

Bara J. E., O’Harra K. E. Recent advances in the design of ionenes: toward convergence with high-performance polymers. Macromolecular Chemistry and Physics. 2019;220(13): 1900078. https://doi.org/10.1002/macp.201900078

Banerjee P., Anas M., Jana S., Mandal T. K. Recent developments in stimuli-responsive poly (ionic liquid) s. Journal of Polymer Research. 2020;27: 1-23. https://doi.org/10.1007/s10965-020-02091-8

Arriaza-Echanes C., Velázquez-Tundidor M. V., Angel-López A., … Coll D. Ionenes as potential phase change materials with self-healing behavior. Polymers. 2023;15(22): 4460. https://doi.org/10.3390/polym15224460

Gennis R. B. Biomembranes: molecular structure and function. New York: Springer-Verlag; 1989. 533 p.

Conde M. M., Conde O., Minones J. M. Interactions between polymers and lipid monolayers at the air/water interface: surface behavior of poly(methyl methacrylate)-cholesterol mixed films. The Journal of Physical Chemistry B. 2019;114: 10774–10781. https://doi.org/10.1021/jp103422g

Published
2025-04-17
How to Cite
Kamorin, D. M., Simagin, A. S., Kazantsev, O. A., Slivkin, A. I., Zamyshlyayeva, O. G., Emasheva, A. A., & Melnikova, N. B. (2025). Self-assembly of cationic polymers in mixed polymer-lipid monolayers at the liquid-air interface. Kondensirovannye Sredy I Mezhfaznye Granitsy = Condensed Matter and Interphases, 27(2), 217-225. https://doi.org/10.17308/kcmf.2025.27/12863
Section
Original articles