Effect of gas media on substrate specificity and physicochemical characteristics of chromatographically cleaned adsorbed β-glucosidase of pea plants

Abstract

Highly purified preparation of cell wall adsorbed β-glucosidase of pea plants exposed to short term hypoxia
and CO2-media received. By salting with ammonia sulfate and gel chromatography on G-25 the achieved purification
rate for enzyme was 22.3 for plants under hypoxia and 47.6 for under CO2 –media. It was shown that seedlings under
hypoxia and high concentrations of carbon dioxide had no changes in elution rate of enzyme under
chromatographical purification on the column. Under normal plant aeration the enzyme resolved specific
isosuccinimide β-glycoside (IS-glycoside), p-nithrophenyl-β-D-glucopyranoside (pNPG) and did not resolve
galactopyranosides with α- and β-bounds. In contrast CO2-media increased affinity of enzyme to all substrates
including uridine diphosphate glucose (UDPG). It was noted that under oxygen deficit and CO2-media the enzyme
stability of seedlings increased towards hydrogen peroxide and shift of pH optima from 4.8 to 4.6 was observed.
Presence of significant differences in variety of physicochemical properties for cell wall adsorbed and cytoplasmic β-
glucosidase of pea seedlings was shown