High performance liquid chromatography determination of the major amino acids in complex biological objects using phenylisothiocyanate derivatization

Authors

  • Andrey O. Rudenko the post-graduate student of analytic chemistry department of chemical faculty, St. Petersburg state university, e-mail: andrew_r@inbox.ru
  • Ludmila A. Kartsova Dr.Sc.Chem. the professor of organic chemistry department of chemical faculty, St. Petersburg state university, email: kartsova@gmail.com
  • Sergey I. Snarskiy Cand.Sc.Biol. The head of department of veterinary laboratory, St. Petersburg

Keywords:

High performance liquid chromatography, amino acids, pancreas and prostata glands, mixed fodder, a pancreas extract, meat, phenylisothiocyanate.

Abstract

The technique of determination of the major amino acids in complex biological matrixes
(pancreas and prostata glands, mixed fodders and samples of meat) by reversed-phase HPLC with UVdetection
is suggested. The method includes samples hydrolysis with hydrochloric acid and derivatization
of amino acids using phenylisothiocyanate solution in isopropanol. Influence of mobile phase рН on
resolution of amino acids is established. Conditions of acid hydrolysis at stage of sample preparation are
optimised.

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Published

2019-11-27

Issue

Vol. 10 No. 2 (2010): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

High performance liquid chromatography determination of the major amino acids in complex biological objects using phenylisothiocyanate derivatization. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 10(2). https://journals.vsu.ru/sorpchrom/article/view/2041

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