Модификация поверхности каналов микрочипа с электрохимическим детектированием для определения биологически активных веществ
Ключевые слова:
микрочиповый капиллярный электрофорез, микрофлюидные системы, электрохимическое детектирование, электроосмотический поток, ПДМС, анализ нейротрансмиттеров.
Аннотация
Описан процесс изготовления чип-анализатора на основе полидиметилсилоксана.
Изучены возможности обработки поверхности канала чипа атмосферной плазмой и поверхностно
активными модификаторами (ПАВ) - додецилсульфатом натрия (ДДСН) и дезоксихолатом натрия,
используемых в качестве добавок к рабочему 10 мМ боратному буферу. Проведена оценка
изменения скорости электроосмотического потока (ЭОП). На модельной смеси катехоламинов
проведена оценка изменения эффективности и факторов разрешения аналитов после модификации
поверхности каналов.
Скачивания
Данные скачивания пока не доступны.
Литература
1. Lacher, N. A.,Garrison, K. E.,Martin, R. S., Lunte, S. M. Microchip capillary
electrophoresis/electrochemistry // Electrophoresis 2001, 22, 2526–2536.
2. Wang J., Pumera M., Chatrathi M. P., Escarpa A., Konrad R., Griebel A., Dorner W.,
Lowe H. Towards disposable lab-on-a-chip: Poly(methylmethacrylate) microchip
electrophoresis device with electrochemical detection // Electrophoresis 2002, 23, 596–
601.
3. Wu M.H. Simple poly(dimethylsiloxane) surface modification to control cell adhesion
// Surf. Interface Anal. 2009, 41, 11–16.
4.Wang B., Abdulali-Kanji Z., Dodwell E., Horton J.H., Oleschuk R.D. Surface
characterization using chemical force microscopy and the flow performance of modified
polydimethylsiloxane for microfluidic device applications // Electrophoresis 2003, 24,
1442–1450.
5. Leclerc E., Sakai Y., Fujii T. Microfluidic PDMS (Polydimethylsiloxane) bioreactor
for large-scale culture of hepatocytes // Biotechnol. Prog.2004, 20, 750–755.
6. Mehta G., Kiel M.J., Lee J.W., Kotov N., Linderman J.J., Takayama S.Polyelectrolyteclay-
protein layer films on microfluidic PDMS bioreactor surfaces for primary murine
bone marrow culture // Adv. Funct. Mater. 2007, 17, 2701–2709.
7. Zhang Q., Xu J. J., Chen H. Y. Patterning microbeads inside poly(dimethylsiloxane)
microfluidic channels and its application for immobilized microfluidic enzyme reactors //
Electrophoresis 2006, 27, 4943–4951.
8. Hashimoto M., Barany F., Soper S.A. Polymerase chain reaction/ligase detection
reaction/hybridization assays using flow-through microfluidic devices for the detection of
low-abundant DNA point mutations // Biosens. Bioelectron. 2006, 21, 1915–1923.
9. Liu Y.J., Rauch C.B. DNA probe attachment on plastic surfaces and microfluidic
hybridization array channel devices with sample oscillation // Anal. Biochem. 2003, 317 ,
76–84.
10. Liu C., Li J.-M., Liu J.-S., Wang L.-D., Hao Z.-X., Chen H.-W. Fracture mechanism
of metal electrode integrated on a chip and fabrication of a poly(ethylene terephthalate). //
Talanta 2009, 79 (5) , pp. 1341-1347
11.Liu A.-L., He F.-Y., Hu Y.-L., Xia X.-H. Plastified poly(ethylene terephthalate)
(PET)-toner microfluidic chip by direct-printing integrated with electrochemical detection
for pharmaceutical analysis.// Talanta 2006, 68 (4) , pp. 1303-1308.
12. Nielsen T., Nilsson D., Bundgaard F., Shi P., Szabo P., Geschke O., Kristensen A..
Nanoimprint lithography in the cyclic olefin copolymer, Topas, a highly UV-transparent
and chemically resistant thermoplast // J. Vac. Sci. Technol. 2004. B. 22. P. 1770-1775.
13. Lacher N.A., Garrison K.E., Martin R.S., Lunte S.M. Microchip capillary
electrophoresis / electrochemistry // Electrophoresis. 2001. V. 22. P. 2526–2536.
14. Vandaveer W.R., Pasas S.A., Martin R.S., Lunte S.M. Recent developments in
amperometric detection for microchip capillary electrophoresis // Electrophoresis. 2002. V.
23. P. 3667–3677.
15. Martin R.S., Gawron A.J., Lunte S.M., Henry C.S. Dual electrode detection on
poly(dimethylsiloxane)-fabricated microchips // Anal. Chem. 2000. V. 72. P. 3196–3202.
16. Lacher N.A., Lunte S.M., Martin R.S. Development of a Microfabricated Palladium
Decoupler/Electrochemical Detector for Microchip Capillary Electrophoresis Using a
Hybrid Glass/Poly(dimethylsiloxane) Device. // Anal. Chem. 2004. V. 76. P. 2482–2491
17. Bruin G.J.M.. Recent developments in electrokinetically driven analysis on
microfabricated devices // Electrophoresis. 2000. V. 21. P. 3931–3951.
18. Cannon D.M., Kuo T.C., Bohn P.W., Sweedler J.V. Nanocapillary array
interconnects for gated analyte injections and electrophoretic separations in multilayer
microfluidic architectures // Anal. Chem. 2003, 75, 2224–2230.
19. Bodas D., Khan-Malek C. Hydrophilization and hydrophobic recovery of PDMS by
oxygen plasma and chemical treatment-An SEM investigation // Sens. Actuator B Chem.
2007, 123 , 368–373.
20. Ren X., Bachman M., Sims, C., Li, G. P., Allbritton, N., J. Electroosmotic properties
of microfluidic channels composed of poly(dimethylsiloxane) // Chromatogr. B 2001, 762 , 117–125.
21. Badal M.Y., Wong M., Chiem N., Salimi-Moosavi, H., Har-rison, D. J., Protein
separation and surfactant control of electroosmotic flow in poly(dimethylsiloxane)-coated
capillaries and microchips // J. Chromatogr. A 2002, 947 , 277–286.
electrophoresis/electrochemistry // Electrophoresis 2001, 22, 2526–2536.
2. Wang J., Pumera M., Chatrathi M. P., Escarpa A., Konrad R., Griebel A., Dorner W.,
Lowe H. Towards disposable lab-on-a-chip: Poly(methylmethacrylate) microchip
electrophoresis device with electrochemical detection // Electrophoresis 2002, 23, 596–
601.
3. Wu M.H. Simple poly(dimethylsiloxane) surface modification to control cell adhesion
// Surf. Interface Anal. 2009, 41, 11–16.
4.Wang B., Abdulali-Kanji Z., Dodwell E., Horton J.H., Oleschuk R.D. Surface
characterization using chemical force microscopy and the flow performance of modified
polydimethylsiloxane for microfluidic device applications // Electrophoresis 2003, 24,
1442–1450.
5. Leclerc E., Sakai Y., Fujii T. Microfluidic PDMS (Polydimethylsiloxane) bioreactor
for large-scale culture of hepatocytes // Biotechnol. Prog.2004, 20, 750–755.
6. Mehta G., Kiel M.J., Lee J.W., Kotov N., Linderman J.J., Takayama S.Polyelectrolyteclay-
protein layer films on microfluidic PDMS bioreactor surfaces for primary murine
bone marrow culture // Adv. Funct. Mater. 2007, 17, 2701–2709.
7. Zhang Q., Xu J. J., Chen H. Y. Patterning microbeads inside poly(dimethylsiloxane)
microfluidic channels and its application for immobilized microfluidic enzyme reactors //
Electrophoresis 2006, 27, 4943–4951.
8. Hashimoto M., Barany F., Soper S.A. Polymerase chain reaction/ligase detection
reaction/hybridization assays using flow-through microfluidic devices for the detection of
low-abundant DNA point mutations // Biosens. Bioelectron. 2006, 21, 1915–1923.
9. Liu Y.J., Rauch C.B. DNA probe attachment on plastic surfaces and microfluidic
hybridization array channel devices with sample oscillation // Anal. Biochem. 2003, 317 ,
76–84.
10. Liu C., Li J.-M., Liu J.-S., Wang L.-D., Hao Z.-X., Chen H.-W. Fracture mechanism
of metal electrode integrated on a chip and fabrication of a poly(ethylene terephthalate). //
Talanta 2009, 79 (5) , pp. 1341-1347
11.Liu A.-L., He F.-Y., Hu Y.-L., Xia X.-H. Plastified poly(ethylene terephthalate)
(PET)-toner microfluidic chip by direct-printing integrated with electrochemical detection
for pharmaceutical analysis.// Talanta 2006, 68 (4) , pp. 1303-1308.
12. Nielsen T., Nilsson D., Bundgaard F., Shi P., Szabo P., Geschke O., Kristensen A..
Nanoimprint lithography in the cyclic olefin copolymer, Topas, a highly UV-transparent
and chemically resistant thermoplast // J. Vac. Sci. Technol. 2004. B. 22. P. 1770-1775.
13. Lacher N.A., Garrison K.E., Martin R.S., Lunte S.M. Microchip capillary
electrophoresis / electrochemistry // Electrophoresis. 2001. V. 22. P. 2526–2536.
14. Vandaveer W.R., Pasas S.A., Martin R.S., Lunte S.M. Recent developments in
amperometric detection for microchip capillary electrophoresis // Electrophoresis. 2002. V.
23. P. 3667–3677.
15. Martin R.S., Gawron A.J., Lunte S.M., Henry C.S. Dual electrode detection on
poly(dimethylsiloxane)-fabricated microchips // Anal. Chem. 2000. V. 72. P. 3196–3202.
16. Lacher N.A., Lunte S.M., Martin R.S. Development of a Microfabricated Palladium
Decoupler/Electrochemical Detector for Microchip Capillary Electrophoresis Using a
Hybrid Glass/Poly(dimethylsiloxane) Device. // Anal. Chem. 2004. V. 76. P. 2482–2491
17. Bruin G.J.M.. Recent developments in electrokinetically driven analysis on
microfabricated devices // Electrophoresis. 2000. V. 21. P. 3931–3951.
18. Cannon D.M., Kuo T.C., Bohn P.W., Sweedler J.V. Nanocapillary array
interconnects for gated analyte injections and electrophoretic separations in multilayer
microfluidic architectures // Anal. Chem. 2003, 75, 2224–2230.
19. Bodas D., Khan-Malek C. Hydrophilization and hydrophobic recovery of PDMS by
oxygen plasma and chemical treatment-An SEM investigation // Sens. Actuator B Chem.
2007, 123 , 368–373.
20. Ren X., Bachman M., Sims, C., Li, G. P., Allbritton, N., J. Electroosmotic properties
of microfluidic channels composed of poly(dimethylsiloxane) // Chromatogr. B 2001, 762 , 117–125.
21. Badal M.Y., Wong M., Chiem N., Salimi-Moosavi, H., Har-rison, D. J., Protein
separation and surfactant control of electroosmotic flow in poly(dimethylsiloxane)-coated
capillaries and microchips // J. Chromatogr. A 2002, 947 , 277–286.
Опубликован
2019-11-20
Как цитировать
Nikolaev, A. V., & Kartsova, L. A. (2019). Модификация поверхности каналов микрочипа с электрохимическим детектированием для определения биологически активных веществ. Сорбционные и хроматографические процессы, 13(1). извлечено от https://journals.vsu.ru/sorpchrom/article/view/1598
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