Экспресс-анализ ацетона в выдыхаемом воздухе при диагностике сахарного диабета
Аннотация
Анализ выдыхаемого воздуха является активно развивающейся областью медицинской неинвазивной диагностики, поскольку такой вид анализа не предполагает инвазивных вмешательств и может проводиться многократно. Известно, что ацетон, содержащийся в выдыхаемом воздухе, коррелирует с уровнем глюкозы в крови и является биомаркером диабета. Использование в медицинских учреждениях аналитических систем для анализа ацетона в выдыхаемом воздухе позволит своевременно диагностировать патологические изменения уровня глюкозы в крови, проводить раннюю диагностику диабета и вести мониторинг эффективности терапии.
В настоящей работе показан экспресс-анализ ацетона в выдыхаемом воздухе с использованием мобильного диагностического комплекса на основе микрофлюидных систем, включающего газовый микрохроматограф «ПИА с термохимическим детектором, планарной хроматографической колонкой, термостатируемой системой пробоотбора и селективного улавливания мешающих компонентов, управляемого электрическими микроклапанами, автоматической системой дозирования с регулируемой продувкой от 0.1 до 5 сек и программным обеспечением. Предложена методика проведения анализа в диагностически значимом диапазоне концентраций: от 0.5 ppm до 20 ppm ацетона в выдыхаемом воздухе при диагностике диабета. Общее время анализа составляет 3 мин, время удерживания для ацетона 60 сек. Диагностический комплекс апробирован и позволяет напрямую определить ацетон в выдыхаемом воздухе. Применение системы улавливания и осушения демонстрирует отсутствие потери целевого вещества и позволяет добиться хорошей сходимости результатов, отклонение от опорного значения не превышает 10%. Апробация в натурных условиях подтвердила данные, полученные на модельных смесях. Разработанный мобильный диагностический комплекс с автоматическим программируемым дозированием и методика количественного определения ацетона в ВВ (0.5-20 ppm) может быть рекомендована к использованию в клинических исследованиях в условиях медицинских учреждений
Скачивания
Литература
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