Adsorption of eremomycin on the walls of fused silica and modified capillaries in the process of electrophoretic separation of aromatic acid enantiomers

Authors

  • Margarita V. Lebedeva postgraduate student (1st year of studies), Analytical Chemistry division, Chemistry Department, M.V. Lomonosov Moscow State University, Moscow, e-mail: margoL87@mail.com
  • Aleksandra F. Prokhorova junior researcher, Analytical, Chemistry Department, M.V. Lomonosov Moscow State University, Moscow
  • Elena N. Shapovalova associate professor, Analytical Chemistry division, Chemistry Department, M.V. Lomonosov Moscow State University, Moscow
  • Oleg A. Shigun corresponiding member of Russ. Acad. Sci., Head of the Chromatography Laboratory, Analytical Chemistry division, Chemistry Department, M.V. Lomonosov Moscow State University, Moscow
  • Sergei M. Staroverov Head of the Laboratory of new chemical technologies for medicine, Chemical Enzymology division, Chemistry Department, M.V. Lomonosov Moscow State University, Moscow
  • Mikhail A. Kuznetsov senior researcher, Chemical Enzymology division, Chemistry Department, M.V. Lomonosov Moscow State University, Moscow

Keywords:

coated capillaries, eremomycin, adsorption.

Abstract

Methods for the preparation of fused-silica capillaries coated with chitosan, 3-
aminopropyltrimethoxysilane, 3-glicidoxypropyltriethoxysilane and eremomycin were offered. It was
found that the most stable coating consisted of eremomycin covalently bonded to the silica surface
through 3-glicidoxypropyltriethoxysilane. The adsorption of eremomycin on the capillary surface was
studied. It was shown that capillary coating reduced the eremomycin adsorption. To reduce this
phenomenon, the 3-aminopropyltrimethoxysilane-coated capillary was shown to be more effective than
that with eremomycin immobilized through 3-glicidoxypropyltriethoxysilane. Four profens were
enantioseparated in coated capillaries using as less as 1 mM eremomycin

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Published

2019-11-26

How to Cite

Adsorption of eremomycin on the walls of fused silica and modified capillaries in the process of electrophoretic separation of aromatic acid enantiomers. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 11(5). https://journals.vsu.ru/sorpchrom/article/view/1965

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