Using chromatographic methods for the purification of the key enzymes of the glyoxylate cycle from organisms of different taxonomic groups

Abstract

The article presents an overview of methods for the isolation of key enzymes of the glyoxylate cycle
of organisms from different taxonomic groups using gel chromatography and ion exchange chromatography.
The main interest of the study of the functioning of the glyoxylate cycle enzyme is the study of isocitrate
lyase (ICL), akoninate hydratase (AH) and malate dehydrogenase (MDH). At the first stage, gel filtration
with Sephadex in order to relief from low molecular weight particles is used most often, then goes driven
ion-exchange chromatography on DEAE-cellulose or DEAE-Toyopearl. The final step is using gel column
chromatography with large pore sizes. The process of glyoxylate cycle enzymes purification from organisms
of different taxonomic groups was developed at the Department of Biochemistry and Cell Physiology of
Voronezh State University. This purification scheme allowed us to receive glyoxylate cycle enzymes
preparations (MDH, ICL, AH) with high specific activity