Eluent ability of mobile phases in reversed - phase HPLC and retention of phenolic compounds

Authors

  • Oleg B. Rudakov professor, head of the chair of physics and chemistry of Voronezh state university of architecture and civil engineering, Voronezh
  • Lyudmila V. Rudakova k.kh.n., assistant professor of the chair of pharmaceutical chemistry and clinical pharmacy of Voronezh state medical academy, 394000, Voronezh, robi@vmail.ru
  • Eugeny N. Groshev the chief of еducational fire brigade of the Voronezh institute of the state fire-prevention service of the Ministry of Emergency Measures of Russia, Voronezh

Keywords:

reversed - phase HPLC, phenols, hydrophobility, eluent ability, acetonirile, methanol.

Abstract

Dependence of retention volume of phenolic compounds in conditions of microcolumn reversed
- phase HPLC on their hydrophobility and eluent abilities of mobile phases is discussed. As criterion of
hydrophobility of sorbats the logarithm of distribution between water and 1-octanol, designed with
algorithm BioByte is used. The new criterion of eluent forces and eluotropic line for modifiers reversed
mobile phases is offered.

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Published

2019-11-26

Issue

Vol. 11 No. 5 (2011): Sorbtsionnye i Khromatograficheskie Protsessy

Section

Статьи

How to Cite

Eluent ability of mobile phases in reversed - phase HPLC and retention of phenolic compounds. (2019). Sorbtsionnye I Khromatograficheskie Protsessy, 11(5). https://journals.vsu.ru/sorpchrom/article/view/1971

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