A study of arginine sorption by sulfoethylated chitosan followed by a capillary electrophoresis
Abstract
Diagnostics of various human diseases and pathologies requires developing express and sensitive methods for determining amino acids. In this regard, one of the most promising analysis methods is capillary electrophoresis. It allows both determining and separating amino acids. What is more, it does not require toxic and expensive reagents.
The work is devoted to the development of a method for the electrophoretic determination of amino acids and the study of arginine sorption with a sorbent based on N-(2-sulfoethyl)chitosan cross-linked with glutaric aldehyde with a sulfoethylation degree of 1.0 (SEC 1.0).
The method of electrophoretic separation and amino acids determination (arginine, alanine, aminobutyric acid, asparagine, aspartic acid, valine, histidine, glycine, glutamic acid, lysine, leucine, isoleucine, serine, ornithine, oxyproline, methionine, threonine, tryptophan, phenylalanine, cysteine) was developed using a Kapel-105M capillary electrophoresis system. As a result of the studies, the following conditions for the separation of amino acids were optimised: detection wavelength, temperature, time of hydrodynamic sample injection, pH and the nature of the background electrolyte, and β-cyclodextrin concentration. The developed method allows separating and determining 13 amino acids in their joint presence in the solution and determining all the studied 20 amino acids when present individually in the solution. The values of the determination and detection limits were calculated for the amino acids studied by capillary electrophoresis under optimised conditions.
The effect of the pH of the ammonium-acetate buffer solution on arginine sorption by SEC 1.0 was studied by the method of limited volume at an initial concentration of amino acid of 5∙10-5 mol/dm3 (sorbent mass: 0.05 g, solution volume: 10.0 cm3). It was found that the extraction of the amino acid by the sorbent was maximum at pH 6.0 and was 44%. The introduction of an equimolar (in relation to amino acid) amount of copper (II) ions into the studied solution did not lead to an increase in the degree of arginine extraction. The extraction of the amino acid by SEC 1.0 in its copper form increased with an increase in the pH and reached the maximum value of 85% at pH 9.0. The equilibrium of arginine sorption by a sorbent in a copper form at the given pH value was established within 2 hours of phase contact.
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References
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