Determination of 3-(4-hydroxyphenyl)lactic acid by an amperometric sensor with molecularly imprinted polymers
Abstract
Sepsis is a life-threatening organ dysfunction caused by the dysregulation of the body’s response to infection. If sepsis is not diagnosed early and treated, it can lead to septic shock, multi-organ failure, and death. The diagnosis of sepsis, traditionally relying on clinical findings and detection of aetiologically significant microorganisms in the blood and foci, has been improved in recent years by the search for and implementation of various biomarkers. One promising biomarker of sepsis is 3-(4-hydroxyphenyl)lactic acid (HPLA). In this study, we developed an amperometric sensor modified with a molecularly imprint polymer (MIP) of hydroxyphenyl lactic acid and proved the fundamental possibility of determining HPLA by this sensor in model aqueous solutions. Molecularly imprinted polymers are widely used to separate substances and to produce selective sensors. Among the variety of selective materials, polyimides are of particular interest. Therefore, in this study, we developed MIP sensors imprinted with 4-hydroxyphenyl lactic acid on the basis of copolymer of 1,2,4,5-benzoltetracarboxylic acid with 4,4'-diaminodiphenyloxide. The sensors were prepared in two stages (1st stage at 80°С, 2nd stage at 180°С) by non-covalent imprinting method. High selectivity of PMO-sensors towards target molecules was confirmed. The range of the determined concentrations of the acid was 0.2-0.0002 mg/dm3. The experimentally determined detection limit of 4-hydroxyphenyl lactic acid was 4.5·10-5 mg/dm3.
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