Determination of the content of tocopherols in the mode of reversed-phase chromatography in plant products with a high protein content
Abstract
Tocopherols are one of the most important biologically active components in the composition of food, which enhance the protective functions of the body. They are considered worldwide as effective antioxidants in the food industry. The aim is to investigate the possibility of tandem use of single–matrix and fluorimetric detectors in the analysis of vitamin E (alpha tocopherol) content in plant objects. The objects of the study are cereals with a high protein content: rye flour, whole wheat flour, amaranth flour and quinoa grain. The study was performed on a Shimadzu LC-20 Prominence liquid chromatograph with series-connected detectors, diode-matrix and fluorimetric. Identification of tocopherols on a diode-matrix detector was carried out with a reference wavelength of 292 nm. An excitation wavelength of 295 nm and an emission wavelength of 330 nm were used on a fluorometric detector. The quantitative analysis of the studied samples was carried out using the method of an external standard. The possibility of using a reverse-phase variant of high-performance liquid chromatography for the analysis of tocopherols in high-protein samples of plant origin is shown. The use of tandem detection using fluorimetric and diode-matrix detectors increases the informative value of chromatographic analysis.
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